N-(1-cyanoethyl)formamide

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions. However, use of industrial activated sludge limits reliabilty.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-(1-cyanoethyl)formamide

EC Number:

248-440-4

EC Name:

N-(1-cyanoethyl)formamide

Cas Number:

27394-99-6

Molecular formula:

C4H6N2O

IUPAC Name:

N-(1-cyanoethyl)formamide

Details on test material:

- Name of test material (as cited in study report): N-Formylalaninnitril + residue

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge, industrial

Details on inoculum:

- Source: BASF sewage treatment plant
- Concentration in stock vessel: 5 g/L

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

22 °C

pH:

7.1

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 2 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

Without test substance:

DOC concentration (mg/l) = 0 mg/l

O2 concentration (0 min) = 7.37 mg/l

O2 concentration (30 min) = 5.64 mg/l

O2 concentration (delta) = 1.73 mg/l

Measuring period = 4 min

Respiration (abs.) = 26 mg/1h

Respiration (rel.) = 0.028 % 

 

100 mg/l test substance:

DOC concentration (mg/l) = 47.6 mg/l

O2 concentration (0 min) = 7.79 mg/l

O2 concentration (30 min) = 6.37 mg/l

O2 concentration (delta) = 1.42 mg/l

Measuring period = 4 min

Respiration (abs.) = 21.2 mg/1h

Respiration (rel.) = -18.3 % 

 

1000 mg/l test substance:

DOC concentration (mg/l) = 476 mg/l

O2 concentration (0 min) = 7.76 mg/l

O2 concentration (30 min) = 6.02 mg/l

O2 concentration (delta) = 1.74 mg/l

Measuring period = 4 min

Respiration (abs.) = 26.2 mg/1h

Respiration (rel.) = 0.592 % 

 

2000 mg/l test substance:

DOC concentration (mg/l) = 951 mg/l

O2 concentration (0 min) = 7.68 mg/l

O2 concentration (30 min) = 5.68 mg/l

O2 concentration (delta) = 2.00 mg/l

Measuring period = 4 min

Respiration (abs.) = 30.0 mg/1h

Respiration (rel.) = 15.2 %