N-(1-cyanoethyl)formamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 - 22 Dec 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles. Acceptable for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-(1-cyanoethyl)formamide

EC Number:

248-440-4

EC Name:

N-(1-cyanoethyl)formamide

Cas Number:

27394-99-6

Molecular formula:

C4H6N2O

IUPAC Name:

N-(1-cyanoethyl)formamide

Details on test material:

- Name of test material (as cited in study report): N-Formylalaninnitril + residue, purity about 84%
- Analytical purity: about 84%
- Lot/batch No.: V126/89 Cont. C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, D-2354 Hohenwestedt
- Length at study initiation (length definition, mean, range and SD): 8.2 cm ( 7.2 - 9.4 cm)
- Weight at study initiation (mean and range): 5.8 g (4.2 - 8.9 g)
- Feeding during test: no feeding


ACCLIMATION
- Acclimation period: 3 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Growing feed F/B 50, SSNIFF Spezialdiaeten GmbH, D-4770 Soest; ad libitum
- Feeding frequency:
- Health during acclimation (any mortality observed): no mortality


HOUSING
The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Photoperiod: 16 h light and 8 h dark
- Total hardness: 2.5 mmol/L
- Acid capacity: 5.5 mmol/L
- Oxygen content: > 60% saturation
- pH: ca. 8.0
- Housing and adaption period: about 3 months

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 - 21 °C

pH:

ca. 8.0

Dissolved oxygen:

7.0 - 8.2 mg/L

Nominal and measured concentrations:

Test concentrations (nominal): 100, 215, 464 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All-glass aquarium (30 x 22 x 24 cm); 10 L test volume
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 5.8 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412 (part 11)
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1


OTHER TEST CONDITIONS
- Photoperiod: 16:8; light: darkness


TEST CONCENTRATIONS
- Spacing factor for test concentrations: about 2.2
- Range finding study
- Test concentrations: 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

215 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

460 - 1 000 mg/L

Results with reference substance (positive control):

- LC50 after 48 h: ca. 34 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

100 and 215 mg/l of the test substance (nominal concentration) didn't result in mortality after 1, 4, 24, 48, 72 or 96 hours. Treatment with 464 mg/l resulted in 1 dead animal after 1h. At this concentration, tumbling was observed after 96 hours. After treatment with 1000 mg/l, 2 animals were dead after 48 hours, all fish were dead after 72 hours. At this concentration, hyperreflexia was observed after 48 hours.

 

In summary, the following results were obtained:

LC50 (mg/l; nominal concentrations)after

1h: >1000

4h: >1000

24h: >1000

48h: >1000

72h: >460, <1000

96h: >460, <1000 

LC0 (48h): 464 mg/l

NOEC: 215 mg/l

 

Maximum concentration tested causing no mortality: 215 mg/l Minimum concentration tested causing 100% mortality: 1000 mg/l