N,N-bis(3-aminopropyl)methylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No GLP, no guideline was indicated (but similar to OECD guideline 403)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N,N-bis(3-aminopropyl)methylamine

EC Number:

203-336-8

EC Name:

N,N-bis(3-aminopropyl)methylamine

Cas Number:

105-83-9

Molecular formula:

C7H19N3

IUPAC Name:

bis(3-aminopropyl)(methyl)amine

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: WIGA, Sulzfeld Germany
- Weight at study initiation: 185 + 15 g
- Diet: Food from Herilan MRH from the firma H . EGGERSMANN KG, Rinteln/Weser
- Water: Tap water ad libitum

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose/head only

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
A dynamic inhalation head - nose exposre system. An infusion pump (UNITA i, B. BRAUN, Melsungen) that constantly assist in supplying aerosols.

TEST ATMOSPHERE
- Brief description of analytical method used: Gaschromatographie
- Samples taken from breathing zone: yes

CLASS METHOD
- Rationale for the selection of the starting concentration: Based of an "inhalation risk test" the doses were selected aimed to include a no effect level (0 mortality).

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Nominal: 0.3, 0.104, 0.1, 0.06 mg/L
Measured (analyt.): 0.12, 0.085, 0.05, 0.035 mg/L

No. of animals per sex per dose:

10 animals per sex per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs and mortality were observed every day. Weighing was performed on day 7 and at the end of the observation period
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology.

Statistics:

Statistics was performed according to D .J . Finney; Probitanalysis 1971, p. 1- 150.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

0.07 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

Mortality was observed at dose groups (nominal) 0.1 mg/L (mortality of male animals 7/20 and 3/20 mortality of female animals, animals died with in a period of 14 days), 0.104 mg/L (mortality of male animals 2/10 and 5/10 mortality of female animlas, animals died within a period of 14 days) , 0.3 mg/L (mortality of male animals 10/10 and 10/10 mortality of female animals, animals died within a period of 8 days).

Clinical signs:

other: Symptoms of exposure observed were clear to reddish eye and nasal discharge, changed breathing pattern, respiratory sounds, reduced movements and squatting posture, staggering and high stepping gait, and ruffled fur.

Body weight:

Body weight development of the female animals exposed to the low concentration was not influenced. Body weight development in the surviving male animals of the other groups was depressed in the first post exposure week in a concentration dependent manner, but reeovered in the second week.

Gross pathology:

During necrapsy the animals that died showed dilation of aurieles, acute general congestion, hyperemia, edema and emphysema of the lungs as well as lobular pattern in the Iiver. Partly carnified lungs were noted in some surviving animals examined at the end of the study.