3-methylcrotonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-methylcrotonic acid

EC Number:

208-782-7

EC Name:

3-methylcrotonic acid

Cas Number:

541-47-9

Molecular formula:

C5H8O2

IUPAC Name:

3-methylbut-2-enoic acid

Test material form:

other: Yellow, cristalline powder

Details on test material:

Purity: 98.7 % , Storage: at 4 °C

Method

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

S-9 fraction (Male Sprague Dawley rats)

Test concentrations with justification for top dose:

6 different doses from 4 µg/ plate to 5000 µg/plate.
0,4,20,100,500, 2500, 5000 µg/plate
Preliminary toxicity testswere performed with all test strains using small number of plates to calculate an appropriate dose range.

Vehicle / solvent:

At the day of the experiment the test substance was dissolved with DMSO at the appropriate concentrations.

Controls

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

N-ethyl-N-nitro-N-nitrosoguanidine

benzo(a)pyrene

other: 2-Aminoanthracene

Details on test system and experimental conditions:

Sufficient S-9 fraction is thawed immediately before each test at room temperature. Bacteria are grown overnight in nutrient broth at 37 °C. Male Sprague Dawley rats (200-300g) received a single intraperitoneal injection of Aroclor 1254 (500 mg/kg bw) 5 days before sacrafice.

Results and discussion

Additional information on results:

Control plates without mutagen showed that the number of spontaneous revertant colonies was similar to that described in the literature. All the positive control compounds gave the expected increase in the number of revertant colonies.

Applicant's summary and conclusion

Conclusions:

Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dose dependend increase in number of revertants in any of the bacterial strains. Also in the presence of the metabolic system, treatment of the cells did not show an increase in the number of revertant colonies. 3-Methylcrotonic acid is not mutagenic.