Registration Dossier
Registration Dossier
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EC number: 208-782-7 | CAS number: 541-47-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1995-08-16-1996-02-15
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO/DIS 10712
- Principles of method if other than guideline:
- Bacteria Inhibition test with Pseudmonas putida according to DIN 38 412, Part 8 (1991) and according to the draft ISO/DIS 10712 (1994)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-methylcrotonic acid
- EC Number:
- 208-782-7
- EC Name:
- 3-methylcrotonic acid
- Cas Number:
- 541-47-9
- Molecular formula:
- C5H8O2
- IUPAC Name:
- 3-methylbut-2-enoic acid
- Details on test material:
- purity: 98,5 %
Constituent 1
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- As inoculum was the bacterial strain Pseudomonas putida Migula, DSM 50026 used.
Study design
- Total exposure duration:
- 17 h
Test conditions
- Test temperature:
- 22 °C
- Reference substance (positive control):
- yes
- Remarks:
- 2,4-Dichlorophenol at the concentration 30-90 mg/l
Results and discussion
Effect concentrations
- Duration:
- 17 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
Applicant's summary and conclusion
- Conclusions:
- The EC50 was determined to be >1000 mg/l
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