Phenylsuccinic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-04-24 - 2018-05-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Phenylsuccinic acid

EC Number:

211-238-1

EC Name:

Phenylsuccinic acid

Cas Number:

635-51-8

Molecular formula:

C10H10O4

IUPAC Name:

2-phenylbutanedioic acid

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

Molecular formula:
C10H10O4
Molecular Mass:
194.18 g/mol
Characteristics (Physical Appearance):
White crystalline powder
CAS No.:
635-51-8
Batch Number:
123
Purity (Analysed by Sponsor) (HPLC):
100%
Purity (Analysed by Sponsor) (T):
99.90%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analysis of prepared dose formulation was performed. The test item stability in OECD media by a validated analytical method has been established for 72 hours.2-Phenylsuccinic Acid was found stable for 72 hours in OECD media.
The concentration verification of the test solution were carried out at 0 hour and 72 hours after initiation of exposure for the concentration of 100 mg/L.

Test solutions

Vehicle:

yes

Details on test solutions:

100 mg of 2-Phenylsuccinic Acid was suspended in acetone to prepare a stock solution of a final volume of 10 ml, with a nominal concentration of 10 mg/ml. This stock was suitably added to test containers to attain the desired test concentration.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Test species:
Unicellular green alga, Pseudokirchneriella subcapitata (Selenastrum capricornutum)
Justification for selection of species:
Species recommended by OECD Guideline No. 201.
Strain:
ATCC-22662
Source:
Procured from American Type Culture Collection (ATCC) and maintained at INTOX PVT. LTD.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

25.4 to 25.5 0C

pH:

6.06 to 8.09

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

Test Condition:
Static
Culture Medium:
OECD Medium
Test Flasks:
The cultures were maintained and tested in conical flasks with capacity 250 ml each containing 100 ml OECD Medium.
Culture Apparatus:
Incubator maintained at 25.4 to 25.5 0C.
Illumination:
A continuous uniform illumination of 4610 to 6860 Lux, cool white light during the test.
pH of the test solutions:
6.06 to 8.09 (0 hour to 72 hours)
Exposure period:
72 hours
Initial Cell Concentration:
25000 cells/mL

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

biomass

Results with reference substance (positive control):

The EyC50 (72 hours) of reference item, Potassium dichromate in fresh water unicellular green algae Pseudokirchneriella subcapitata is estimated to be 0.46 mg/L with fiducial limits between 0.40 to 0.53 mg/L at 95% confidence level.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The ErC50 (72 hours) value of 2-Phenylsuccinic Acid in fresh water unicellular green alga - Pseudokirchneriella subcapitata is estimated to be greater than 100 mg/L.
The EyC50 (72 hours) value of 2-Phenylsuccinic Acid in fresh water unicellular green alga - Pseudokirchneriella subcapitata is estimated to be greater than 100 mg/L.

Executive summary:

Growth inhibition Test of 2-Phenylsuccinic Acid in fresh water green alga -Pseudokirchneriella subcapitata (Selenastrum capricornutum) was performed in compliance with the OECD Guideline for Testing of Chemicals, No. 201, ‘Freshwater Alga and Cyanobacteria, Growth Inhibition Test’, Section 2, adopted by the Council on 23 March 2006 and Annex 5 corrected: 28 July 2011.

The ErC50 (72 hours) value of 2-Phenylsuccinic Acid in fresh water unicellular green alga - Pseudokirchneriella subcapitata is estimated to be greater than 100 mg/L.

The EyC50 (72 hours) value of 2-Phenylsuccinic Acid in fresh water unicellular green alga - Pseudokirchneriella subcapitata is estimated to be greater than 100 mg/L.