Diethylammonium chloride

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: data from peer-reviewed handbook

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1950

Report date:

1950

Reference 2

Reference Type:

review article or handbook

Title:

No information

Author:

Smyth, H.F. et al.

Year:

1951

Bibliographic source:

Arch. Ind. hyg. occup. med. 4: 119-122

Reference 3

Reference Type:

secondary source

Title:

Industrial Hygiene and Toxicology

Author:

Sutton W.L.

Year:

1962

Bibliographic source:

cited in: Patty's Ind.Hyg. and Toxicol. 2, 2044

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Penetration of rabbit skin is estimated by a technique closely to the one-day cuff method of Draize and associates, using groups of 5 male albino rabbits weighing 2.5 to 3.5 kg. The fur is removed from the entire trunk by clipping, and the dose is retained beneath an impervious plastic film. The animals are immobilized during the 24 hour contact period, after which the film is removed and the rabbits are caged for the subsequent 14-day observation period.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

diethylamine (CAS: 109-89-7)

IUPAC Name:

diethylamine (CAS: 109-89-7)

Test material form:

other: clear liquid

Details on test material:

- Name of test material (as cited in study report): diethylamine

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 3-5 month
- Weight at study initiation: 2.5 - 3.5 kg
- Diet (e.g. ad libitum): Rockland rabbit diet

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 hours

Doses:

0.252, 0.5, 1.0, 2.0 mL/kg bw = 179, 355, 710 and 1420 mg/kg bw (conversion in mg/kg bw based on the density d: 0.71 g/cm3 of the test substance diethylamine)

No. of animals per sex per dose:

5 (m)

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Frequency of weighing: day 0, 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

582 mg/kg bw

Based on:

test mat.

95% CL:

>= 376 - <= 895

Mortality:

1420 mg/kg bw: 5/5, days to death: 1, 1, 1, 1, 1
710 mg/kg bw: 3/5, days to death: 6, 1, 1
355 mg/kg bw: 1/5, days to death: 1
179 mg/kg bw: 0/5

Clinical signs:

Local effects: the substance caused hemorrhage and necrosis of the skin and underlying muscular layers.

Body weight:

Survivors of the 355 and 710 mg/kg bw dose group lost weight.

Gross pathology:

Animals that died: pale or mottled livers, pale, mottled or roughened surfaces of the kidneys, and congested or hemorrhagic intestines. Spleens were darkened to the point of being called black, pancreas congested and testes hemorrhagic.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

CLP: Acute Tox. 3, H311
After dermal administration to rats, the test material diethylamine (CAS: 109-89-7), shows an LD50 of 582 mg/kg bw.