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EC number: 425-380-9 | CAS number: 7397-46-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
DEMB was tested in two in vitro genotoxicity tests.
In the Ames test which was performed under GLP according to OECD 471, four histidine-requiring strains of Salmonella typhimurium (TA1535, TA1537, TA100 and TA98) were tested in two independent experiments. DEMB was applied up to concentrations of 5000 ug/plate in the absence and the presence of S9-mix. DEMB did not induce a dose-related increase in the number of revertant colonies both in the absence and the presence of S9-mix.
Secondly, a chromosome aberration test was performed according to GLP and OECD 473. Here DEMB was tested to induce chromosome aberrations in cultured peripheral human lymphocytes in the presence and absence of a metabolic activation system (S9 - mix). In the absence of S9 -mix DEMB was tested up to 560 ug/ml for a 24 h and for a 48 h treatment time. In the second experiment DEMB was tested up to 750 ug/ml for a 24 h treatment time. In the presence of 1.8% (v/v) S9 -fraction DEMB was tested up to 560 ug/mg for 3 h and in the second experiment DEMB was tested up to 1000 ug/ml for 3 h. Both in the absence and presence of S9-mix DEMB induced a statistically and biologically significant increases in the number of cells with chromosome aberrations but only at the highest, cytotoxic, concentration of 560 ug/ml and in the absence of a dose-response relationship (at the 24 h fixation time). At the 48 h fixation time the cytotoxic, concentration of 560 ug/ml induced a statistically and biologically significant increase in the number of cells with chromosome aberrations in the absence of S9-mix only. In the absence of S9-mix DEMB induced a statistically and biologically significant increase in the number of cells with chromosome aberrations at the highest, cytotoxic, concentration of 750 ug/mL in the absence of a dose-response relationship. In the presenceof S9-mix DEMB did not induce a statistically and biologically significant increase in the number of cells with chromosome aberrations at all concentrations tested. Finally, an increase in the number of cells with chromosome aberrations was observed only at high and cytotoxic concentrations.
Considering the toxic-profile of DEMB this is probably not the result of DNA-compound interaction, but is the result of an aspecific, indirect cell damaging action finally resulting in chromosome aberrations. Therefore, non-clastogenicity of DEMB may be considered.
Based on the results of both studies it is concluded that DEMB is not mutagenic.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
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