diethylmethoxyborane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study. Since the hydrolysis of the substance is slow, the daily renewal of the test solution is considered sufficient to keep the test concentration within an acceptable range. No effects were observed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

425-380-9

EC Name:

-

Cas Number:

7397-46-8

Molecular formula:

C5H13BO

IUPAC Name:

methyl diethylborinate

Details on test material:

- Name of test material (as cited in study report): DEMB
- Analytical purity: 99.4%
- Stability under test conditions: No, hydrolyses slowly
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
Initially, 10 mL of test substance was diluted 1:1 with methanol. This mixture was prepared under nitrogen in a glovebox and stored in a freezer prior to use. Weighed amounts with an average of 400.0 ± 1.2 mg from a DEMB/methanol mixture were dissolved in 2000 mL test medium to provide a DEMB concentration of 100 mg/L. The final concentration of methanol in all test solutions was 100 mg/L.

Test organisms

Test organisms (species):

Cyprinus carpio

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg/L CaCO3

Test temperature:

19.8 - 20.5 °C

pH:

7.3 - 8.0

Dissolved oxygen:

3.4 - 9.2 mg/L

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 4 litres, all-glass
- Aeration: The test media were not aerated during the test.
- Renewal rate of test solution (frequency): Test solutions were renewed daily.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.74 g fish/litre, i.e. 7 fish per 2 litres of test medium.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Milli-Ro water (tap.water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA)
- Culture medium different from test medium: no
- Intervals of water quality measurement: temperature, pH and dissolved oxygen measured daily

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- At 3, 24, 48, 72 and 96 hours following the start of exposure.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: No mortality occured during the 96-hour exposure period. However, fish exposed to concentrations of 1 to 100 mg/L showed slight hypoactivity from 24 hours of exposure till the end of the test.

Reference substance (positive control):

yes

Remarks:

pentachlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- No mortality occurred during the 96-hour exposure period. Further, fish did not show any effect on swimming behaviour or appearance.
- Analytical support was not available as detection of the concentrations tested was not possible with any of the analytical methods available. Hence, no sampling for analysis was performed.
- The final test solutions were all clear and colourless.
- Fresh solutions of DEMB had low O2 concentration on day 3, not expected to affect test results.
- No mortality was observed in the control group.

Results with reference substance (positive control):

The 96h-LC50 was 0.16 mg/L with 95 % fiducial limits of 0.14 and 0.19 mg/L