Phosphonic acid, P-(3-silylpropyl)-, Si,Si,Si-tris(mixed ethoxy and methoxy) derivs., mixed Et and Me diesters

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-08-12 to 1992-01-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline test under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

92/69/EWG, C.1

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

225375-65-5

EC Number:

607-104-0

Cas Number:

225375-65-5

IUPAC Name:

225375-65-5

Details on test material:

- Name of test material (as cited in study report): Na-silicophosphonat
- Physical state: Liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Nominal concentrations (based on rangefinding studies): 50, 100, 464, 1000, 2150, 4640 and 10000 mg/L
- 10000 mg/L was tested in parallel after pH-adjustement

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-The product was added to the test water without any pretreatment

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Strain: Walbaum 1972
- Source: BASF's own breed raised from eggs of Forellenzucht Eusserthal petrihof, Eber soehne, Eusserthal
- Length at study initiation (length definition, mean, range and SD): 6.8 cm (Range 6.0-7.5)
- Weight at study initiation (mean and range, SD): 3.4 g (Range 2.4-4.4)

ACCLIMATION
- Acclimation period: -
- Acclimation conditions (same as test or not): housing conditions were the same as test conditions
- Type and amount of food: sniff spezialdiäten; Growing feed F/B 50; ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: ca. 2.5 mmol/L

Test temperature:

12-13 °C

pH:

7.2

Dissolved oxygen:

10.2-10.4 mg/l

Nominal and measured concentrations:

Nominal concentrations: 50, 100, 464, 1000, 2150, 4640 and 10000 mg/L
- 10000 mg/L was tested in parallel after pH-adjustement

Details on test conditions:

TEST SYSTEM

- Type (delete if not applicable): glass, 100L
- Renewal rate of test solution (frequency/flow rate): not given
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):-
- Biomass loading rate: 0.34 g/l


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Adjustment of pH: only for 10000 mg/L
- Photoperiod: 16/8

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50, NOEC 0, 1, 4, 24, 48, 72 and 96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2 200 - < 4 600 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

2 150 mg/L

Nominal / measured:

nominal

Basis for effect:

behaviour

Effect concentrations 3

Duration:

1 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

4 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 4 600 - < 10 000 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 6

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 2 200 - < 4 600 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 7

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 2 200 - < 4 600 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Details on results:

slight sedimentation at concentrations higher 2150 mg/L

Results with reference substance (positive control):

None used

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance is not toxic to the fish in concentrations under 2150 mg/L. Effects above this concentration could be prevented by pH-adjustment

Executive summary:

To assess the acute toxicity a study according to OECD 203 was performed with Na-silicophosphonat. The concentration range was from 50 to 10000 mg/L. The control worked as expected. The LC50 (96h) was between 2200 and 4600 mg/L. The NOEC was 2150 mg/l.