Reaction mass of 3-(octanoyloxy)propyl decanoate and propane-1,3-diyl didecanoate and propane-1,3-diyl dioctanoate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Oxygen-consumption test according to Hüls AG (Dr. Schöberl)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

The test item was emusified in Nonylphenol plus 10 E0 plus 5 PO.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Test organisms: Pseudomonas putida Migula
- Laboratory culture: from Dr. Reinhard Kanne, Bayer AG, Leverkusen
- Pretreatment: A liquid culture of the test organisms was incubated for 19h, 10 x 10^5 germ/mL, extinction (436 nm): 0.29 - 0.31. 80 mL of this liquid culture was inoculated with 200 mL sterile stocksolution 1 and 2 and 5 minutes aerated and mixed. Extinction of the bacteria suspension: 0.304.
Details on stocksolution 1: 20 g D(+)Glucose, 4.24 g NaNO3 p. a., 2.40 g K2HPO4 (without water), 1.20 g KH2PO4 p. a., 30 mL trace element solution (see below), 1000 mL double destilled water. Sterilise at 100 °C, 40 min with drifty steam.
Trace element solution (all vaues in g per 500 mL double destilled water): 0.0275 Al2 (SO4)3 x 18 H2O, 0.014 KJ, p. a., 0.014 KBr, p. a., 0.0275 TiO2, (Titan IV oxid), 0.014 SnCl2 x 2 H2O, p. a., 0.014 LiCl, p.a., 0.1945 MnCl2 x 4 H2O, p. a., 0.307 H3BO3, 0.0275 ZnSO4 x 7 H2O, p. a., 0.0275 CuSO6 x 5 H2O, p. a., 0.0295 NiSO4 x 6 H2O, 0.0275 Co(NO3)2 x 6 H2O, reinst
Details on stocksolution 2: 0.200 g FeSO4 x 7 H2O p.a., 3.71 g MgSO4 x 6 H2O p.a. fill up to 1L with double destilled water, shake, filter sterile

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

4.75 h

Test temperature:

25.0 - 25.7 °C

Nominal and measured concentrations:

Nominal concentrations: 0.99, 9.9, 99, 990 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: BSB-flask
- Type: closed
- Material, fill volume: glass, 100 mL
- Aeration: no
- No. of vessels per concentration (replicates): 4 (2 for measurements at start, 2 for measurements at end)
- No. of vessels per control (replicates): 5
- No. of vessels per abiotic control (replicates): 4 (2 for measurements at start, 2 for measurements at end)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no details available

EFFECT PARAMETERS MEASURED
oxygen concentration was measured at the start and at the end of the test

Reference substance (positive control):

no

Duration:

4.75 h

Dose descriptor:

EC50

Effect conc.:

> 990 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Key result

Duration:

4.75 h

Dose descriptor:

EC10

Effect conc.:

> 990 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

The test item caused a higher consumption of O2 compared to the control. In the highest concentration of 0.99 g/L the consuption was 13.5% higher. For details please see Table 1 below. Therefore the test item does not inhibit but stimulate the respriation.

Table 1: Details on results

Concentration (mg/L)	Inhbition of respiration (%)
0.99	-4.4
9.9	-3.7
99	-8.7
990	-13.5

Validity criteria fulfilled:

yes

Conclusions:

In the respiration inhibition test the EC10 was >0.99 g/L.

Executive summary:

In the oxygen-consumption test according to Hüls AG (Dr. Schöberl) the respiration inhibition of the test substance was determined. Four concentrations of 0.99, 9.9, 99 and 990 mg/L were used. After incubation of 4.75 h with Pseudomonas putida Migula the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 0.99 g/L.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Version / remarks:

EG-Richtlinie-Nr.: 88/302/EG (30.5.1988)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test item was pipetted directly in the test vessels.

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Name and location of sewage treatment plant where inoculum was collected: mainly domestic wastwater treatment plant "Marl-West"
- Pretreatment: On the day of sampling the sewage was settled twice and washed with 1-2 L drinking water. Afterwards 200 mL were filled up to 4 L with drinking water and stored aerated and stirred. On the start of the experiment it was washed twice with drinking water. The final activated sludge had a dry content of 3.79 g/L

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test temperature:

20 ± 2 °C

pH:

8.1 - 8.5

Nominal and measured concentrations:

Nominal concentrations: 45.8, 91.6, 183.2, 366.4 and 1099 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: vessels
- Type: open
- Material, fill volume: glass, 500 mL
- Aeration: yes (0.5 - 1 L/min)
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2
- No. of vessels per reference (replicates): 3

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no details available

EFFECT PARAMETERS MEASURED
oxygen concentration was measured at the start and at the end of the test

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

The test item caused a higher consumption of O2 compared to the control. In the highest concentration of 1.1 g/L the consuption was 17.9% higher. For details please see Table 1 below. Therefore the test item does not inhibit but stimulate the respriation.

Results with reference substance (positive control):

Reference substance: 3,5 Dichlorophenol (5, 15, 30 mg/L)
EC50-limit: 5-30 mg/L
EC50-determined: 8.7 mg/L

Table 1: Details on results

Substance	Concentration (mg/L)	Inhbition of respiration (%)
Test item	45.8	-17.3
	91.6	-15.3
	183.4	-17.4
	366.4	-18.7
	1100	-17.9
Reference substance	5	38.4
	15	58.0
	30	80.7

Validity criteria fulfilled:

yes

Conclusions:

In the respiration inhibition test the EC10 was >1.1 g/L.

Executive summary:

In the oxygen-consumption test according to OECD 209 the respiration inhibition of the test substance was determined. The concentrations of 45.8, 91.6, 183.2, 366.4 and 1099 mg/L were used. After incubation of 3 h with activated sludge the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 1.1 g/L.

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to Chapter 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

4.75 h

Dose descriptor:

EC10

Effect conc.:

> 990 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

4.75 h

Dose descriptor:

EC50

Effect conc.:

> 990 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Reference 4

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to Chapter 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Description of key information

No data on the test item is available. With the read-across substances the following results were obtained:

CAS 68583-51-7: EC10 > 0.9 g/L, NOEC = 0.9 g/L

CAS 853947-59-8: EC10 > 1.1 g/L, NOEC = 1.1 g/L

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

900 mg/L

Additional information

No data on the test item is available. With the read-across substances the following results were obtained:

In the oxygen-consumption test according to Hüls AG (Dr. Schöberl) the respiration inhibition of the read across substance CAS 68583-51-7 was determined. Four concentrations of 0.99, 9.9, 99 and 990 mg/L were used. After incubation of 4.75 h with Pseudomonas putida Migula the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 0.99 g/L.

In the oxygen-consumption test according to OECD 209 the respiration inhibition of the read across substance CAS 853947-59-8 was determined. The concentrations of 45.8, 91.6, 183.2, 366.4 and 1099 mg/L were used. After incubation of 3 h with activated sludge the respiration of the organisms was stimulated and not inhibited. Therefor the EC10 and EC50 > 1.1 g/L.