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EC number: 684-597-9 | CAS number: 1072005-10-7
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.04. to 22.05.1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum: wastewater treatment plant Marl-Ost, sampled on 22.04.1997. The sludge was reconditioned as suggested by the guideline. The bacteria in the inoculum were determined with the pour-plate method of Koch to be 125 * 10^4 colonbilding units/mL.
- Duration of test (contact time):
- 29 d
- Initial conc.:
- > 21 - < 21.1 mg/L
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium, no details available
- Test temperature: 20.6 - 22.8 °C
- pH: 7.4 - 7.6
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 27.7 mg/L
- dry content of inoculum: 4.2 g/L
TEST SYSTEM
- Culturing apparatus: glass flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration
- Measuring equipment: carbon content analyser
SAMPLING
- Sampling frequency: On day 0, 2, 5, 8, 14, 19, 23 and 28
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Toxicity control: 1 flasks - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 87
- Sampling time:
- 28 d
- Details on results:
- After 2 days, the biodegradation of the test item was 6% and after 5 days 52%. Thereafter the biodegration rose steadily until 87% on day 28. The 10-day window was passed.
- Results with reference substance:
- The reference substance schowed good biodegradation with 90% after 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is readily biodegradable (87 % in 28 days).
- Executive summary:
The biodegradation of the test item was determined in a modified sturm test according to EU method C.4 -C. The test substance is inoculated with activated sludge of a domestic WWTP and incubated for 28 days under aerobic conditions in the dark at 20 - 22 °C. During this period, the biodegradation of the test substance is determined on the basis of the generation of CO2. Within the test period of 28 days, a degradation of 87 % was determined. The degradation of the toxicity control was 90 % after 28 days.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12.07. - 10.08.1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum: wastewater treatment plant Marl-Ost, sampled on 11.07.1995. The sludge was reconditioned as suggested by the guideline. The bacteria in the inoculum were determined with the pour-plate method of Koch to be 102 * 10^4 colonbilding units/mL.
- Duration of test (contact time):
- 29 d
- Initial conc.:
- 21.16 mg/L
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium, no details available
- Test temperature: 22.2 - 24.2 °C
- pH: 7.6 - 7.7
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 28 mg/L
- dry content of inoculum: 4.2 g/L
TEST SYSTEM
- Culturing apparatus: glass flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration
- Measuring equipment: carbon content analyser
SAMPLING
- Sampling frequency: On day 0, 2, 5, 8, 14, 19, 23 and 28
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks
- Toxicity control: 1 flasks - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82
- Sampling time:
- 28 d
- Details on results:
- On one day the temperature in the test room was above 24 °C. As the control substance showed normal biodegradation, the effect on the organisms can be neglected.
After 2 days, the biodegradation of the test item was 19% and after 5 days 55%. Thereafter the biodegration rose steadily until 82% on day 28. The 10-day window was passed. - Results with reference substance:
- The reference substance schowed good biodegradation with 88% after 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is readily biodegradable (82 % in 28 days).
- Executive summary:
The biodegradation of the test item was determined in a modified sturm test according to EU method C.4 -C. The test substance is inoculated with activated sludge of a domestic WWTP and incubated for 28 days under aerobic conditions in the dark at 22 - 24 °C. During this period, the biodegradation of the test substance is determined on the basis of the generation of CO2. Within the test period of 28 days, a degradation of 82 % was determined. The degradation of the toxicity control was 88 % after 28 days.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 87
- Sampling time:
- 28 d
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 82
- Sampling time:
- 28 d
Referenceopen allclose all
Table 1: Details on results
Duration (days) |
Biodegradation of the test substance (mean of two replicates, %) |
Biodegradation of reference substance (%) |
0 |
0 |
0 |
2 |
6 |
43 |
5 |
52 |
76 |
8 |
66 |
85 |
14 |
80 |
94 |
19 |
83 |
92 |
23 |
85 |
94 |
28 |
87 |
90 |
Table 1: Details on results
Duration (days) |
Biodegradation of the test substance (mean of two replicates, %) |
Biodegradation of reference substance (%) |
0.02 |
0 |
0 |
2 |
19 |
45 |
5 |
55 |
70 |
8 |
69 |
80 |
14 |
78 |
85 |
19 |
81 |
85 |
23 |
80 |
88 |
28 |
82 |
88 |
Description of key information
No data on the test item is available. With the read-across substances the following results were obtained:
CAS 68583-51-7: Biodegradation (28d): 82%
CAS 853947-59-8: Biodegradation (28d): 87%
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
No data on the test item is available. With the read-across substances the following results were obtained:
The biodegradation of the read across substance CAS 68583-51-7 was determined in a modified sturm test according to EU method C.4 -C. The test substance is inoculated with activated sludge of a domestic WWTP and incubated for 28 days under aerobic conditions in the dark at 22 - 24 °C. During this period, the biodegradation of the test substance is determined on the basis of the generation of CO2. Within the test period of 28 days, a degradation of 82 % was determined. The degradation of the toxicity control was 88 % after 28 days.
The biodegradation of the read across substance CAS 853947-59-8 was determined in a modified sturm test according to EU method C.4 -C. The test substance is inoculated with activated sludge of a domestic WWTP and incubated for 28 days under aerobic conditions in the dark at 20 - 22 °C. During this period, the biodegradation of the test substance is determined on the basis of the generation of CO2. Within the test period of 28 days, a degradation of 87 % was determined. The degradation of the toxicity control was 90 % after 28 days.
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