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EC number: 615-206-1
CAS number: 709031-45-8
The study was performed
to assess the skin sensitization
potential of BMS 482204-03 (MSA salt)
using the murine
local lymph node
The study was
performed in compliance with:
of Chemicals No. 429 "Skin Sensitization:
Local Lymph Node Assay".
(24 April 2002).
EPA Health Effects
ICCVAM (Interagency Co-ordinating Committee
on the Validation
local lymph node assay: A
for assessing the
allergic contact dermatitis potential of chemicals/compounds. NIH Publication
No. 99-4494, National
Institute of Environmental
Health Sciences, Research Triangle Park, North
Carolina, USA. The
procedure followed was based on the method
KIMBER I., HILTON
J. and WEISENBERGER
C. (1989). The murine
local lymph node assay for
identification of contact allergens: A
preliminary evaluation of in situ measurement
of lymphocyte proliferation. Contact
Dermatitis 21, 215-220.
D.A. and SCHOLES E.W. (1992). Comparison
of the local lymph node
assay with the
guinea-pig maximisation test for the detection of a
range of contact allergens. Food
Toxicology 30, 65-69
In this study, four
five mice were treated
as follows: Control (Acetone:Olive Oil 4:1 v/v), 5%, 10% and 25 w/v
Each group of mice was
treated by daily application of 25µl of each of the respective test or
material, to the dorsal surface of both ears for three consecutive days. The
proliferative response of the lymph node cells (LNC) from the draining
auricular lymph nodes was
assessed five days
following the initial
measurement of the incorporation of
by fl-scintillation counting of LNC suspensions. The
response was expressed as radioactive disintegrations per minute per
lymph node (dpm/node) and as the ratio of 3HTdR incorporation into LNC
oftest nodes relative to that recorded for control nodes (test/control
ratio). The test substance
is regarded as a
sensitizer if at least one concentration
of the chemical results in a
three-fold greater increase in 3HTdR incorporation compared to the vehicle control values.
In this assay the test/control
ratios obtained for 5, 10
and 25 %
w/v were 1.4, 1.8 and 1.5
respectively which indicates that BMS 482204-03
(MSA salt) did not show
(delayed contact hypersensitivity).
to the positive control substance hexyl cinnamic aldehyde (HCA), in a
contemporaneous study (addendum) demonstrate the reliability and
sensitivity of this assay to detect skin sensitization potential in this
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