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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin sensitisation, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report date:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: KIMBER 2001
GLP compliance:
yes
Type of study:
other: THP-1 cells, Acute Monocytic Leukema

Test material

Constituent 1
Reference substance name:
Hydrocotyle asiatica, ext.
EC Number:
283-640-5
EC Name:
Hydrocotyle asiatica, ext.
Cas Number:
84696-21-9
Molecular formula:
not applicable
IUPAC Name:
Hydrocotyle asiatica, ext.
Test material form:
solid

Results and discussion

In vitro / in chemico

Resultsopen allclose all
Key result
Run / experiment:
other: 1: 100 microg/mL
Parameter:
other: Optical density
Value:
1.84
Negative controls validity:
valid
Key result
Run / experiment:
other: 2: 100 microg/mL
Parameter:
other: Optical density
Value:
1.91
Negative controls validity:
valid
Key result
Run / experiment:
other: 3: 100 microg/mL
Parameter:
other: Optical density
Value:
2.071
Negative controls validity:
valid
Key result
Run / experiment:
other: 4: 10 microg/mL
Parameter:
other: Optical density
Value:
2.505
Negative controls validity:
valid
Key result
Run / experiment:
other: 5: 10 microg/mL
Parameter:
other: Optical density
Value:
2.012
Negative controls validity:
valid
Key result
Run / experiment:
other: 6: 10 microg/mL
Parameter:
other: Optical density
Value:
2.168
Negative controls validity:
valid
Key result
Run / experiment:
other: 7: 1 microg/mL
Parameter:
other: Optical density
Value:
2.642
Negative controls validity:
valid
Key result
Run / experiment:
other: 8: 1 microg/mL
Parameter:
other: Optical density
Value:
2.386
Negative controls validity:
valid
Key result
Run / experiment:
other: 9: 1 microg/mL
Parameter:
other: Optical density
Value:
2.363
Negative controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Centella extract at the concentration of 100 microg / mL is not sensitizing.
Due to the fast cellular growth of the THP-1 seeded for MTT test, the optical density of the wells treated with centella 1-10 micro/g mL were out of the accepted range (0-2 OD) and have not been considered.