Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to current OECD-Guidelines and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate
EC Number:
228-528-9
EC Name:
Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate
Cas Number:
6289-46-9
Molecular formula:
C10H12O6
IUPAC Name:
1,4-dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Neonates of Daphnia magna, less than 24 hours old, were obtained by hatching ephippia,
supplied in the microbiotest "Daphtoxkit F 'M "(Fa. BioMicrotests, lndustriezone "De Prijkels",
Venacoweg 19, 9810 Nazareth, Belgium). Batch No. of the ephippia: DM090403.
The ephippia were hatched in a petri dish containing reconstituted water in a temperature
controlled room at approx. 20-22 "6 under continuous illumination of approximately 6 000
Lux. According to Fa. MicroBioTests the largest hatching occurs between 72 and 80 hours
and the daphnids must be collected at the latest 90 hours after initiation of hatching.
Approximately 2 hours before beginning of the test the neonates were fed with a suspension
of Spirulina powder (Fa. MicroBioTests), as also in traditional cultures the neonates have the
opportunity to take up some feed pat"ricles. The neonates were collected for the test at
approximately 86 hours after initiation of hatching.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
water hardness was 250 mg CaCO3/L.
Test temperature:
20-22 °C
pH:
7.8 - 8.0
Dissolved oxygen:
8.2 mg/L
Nominal and measured concentrations:
25.2 mg test substance to 250 ml

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
not subject of classification. EC50 > 100 mg/l