Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1992

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate

EC Number:

228-528-9

EC Name:

Dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate

Cas Number:

6289-46-9

Molecular formula:

C10H12O6

IUPAC Name:

1,4-dimethyl 2,5-dioxocyclohexane-1,4-dicarboxylate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22 °C

pH:

7,4-8,1

Dissolved oxygen:

> 80% of the saturation value

Nominal and measured concentrations:

The test substance was made into a paste using Tween 80 as solubilizing agent. Water for dilution was added and the mixture homogenized using an Ultra-Turrax and ultrasonic bath and poured into the chamber under stirring with a glass rod. The chambers contents were then stirred for approximately 2 hours with a KPG-stirrer and a glass rod. The 220 mg/l test batch was present as clear solution. The 500 mg/l test batch showed a slight tubidity. Additionally substance deposits on the surface and on the buttom of the chamber were observed. In order to dtermine the substance concentration of the 500 mg/l group, water samples were taken from the middle of the test chamber from the first and third preparation of the test after 0 and 24 hours using a screw-neck, glass bottle. The analytical results were in a range of about 27,9 - 33,5 % of the nominal concentration.

Details on test conditions:

The studdy was conducted in a semistatic system. The fish were transferred each 24hours to new test chambers, which were calibrated to 10 liters, were made out of glass (length 30 cm, width 22 cm, height 24 cm) and stood in a water bath made out of HOSTALIT Z (R) with a Plexiglass viewing panel. The temperature of the water bath was regulated by a thermostat to 22 *_1 °C. The chambers were illuminated from above from 6.00 a.m. to 6.00 p.m.. The light intensity directly over the chambers was approximately 700 lux. The test chambers were not aerated during the course of the study.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Details on results:

actual concentration exceeds 500 mg/l (nominal conc) and 156,5 mg/l (actual conc.).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

no subject of classification.

Executive summary:

The LC50 value for this substance in this OECD 203 test (Brachydanio rerio) is greater than 500 mg / L after 96 hours