Registration Dossier
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EC number: 258-061-6 | CAS number: 52636-67-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The test item can be considered to be not ready biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days in closed bottles.
The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item.
Additionally a toxicity control has been conducted containing both, the test item and the reference item. The dissolved oxygen concentration was measured to calculate the percentage BOD. Furthermore because of the Nitrogen content of the test item, samples for nitrate and nitrite analysis were taken from all vessels (of test item, inoculum control and toxicity control group) and the oxidized Nitrogen (nitrate and nitrite) concentrations were measured after each oxygen measurement.
The reference item Sodium benzoate was sufficiently degraded to a mean of 76.1 % after 14 days, and to a mean of 88.0 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum.
In the toxicity control a mean of 39.1 % biodegradation was noted within 14 days and 41.4 % biodegradation after 28 days of incubation.
Thus, the test item can be assumed to not inhibit the activated sludge microorganisms (higher than 25 % degradation occurred within 14 days).
Under the test conditions the percentage biodegradation of Morpholinium Sulphamate reached a mean of 7.3% after 28 days based on ThODNO3.Therefore the test item can be considered to be not ready biodegradable.
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