Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Reported 12Jun1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate

EC Number:

269-503-2

EC Name:

Methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate

Cas Number:

68259-00-7

Molecular formula:

C14H16N3.CH3O4S

IUPAC Name:

methyl 1-methyl-4-[(methylphenylhydrazono)methyl]pyridinium sulphate

Test material form:

solid: particulate/powder

Details on test material:

See study reports for batch and purity information

Specific details on test material used for the study:

Purity ca 90%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/1, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.

Test solutions

Vehicle:

yes

Details on test solutions:

The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/1, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.
During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The organism used in this test system is the species Daphnia magna. The Daphnia were bred in the laboratories of the Product Ecology TF, Textile Dyes Division under standardised conditions.
Breeding :
Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22''C. The water was renewed partially three times per week. The Daphnia were fed with a suspension of green algae {Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantities that the food was consumed within 24h.

Pre-treatment:
24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 1000 µm sieve.

For each test concentration, 20 Daphnia divided in two groups of 10 animals were used.

Study design

Test type:

static

Water media type:

not specified

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Reference substance (positive control):

yes

Remarks:

The quality of the Daphnia will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

1.04 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

2.9 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

4.4 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.04 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.6 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

4.4 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 7

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1.04 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 (issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/L, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.

During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 1.04 mg/l, the EC100 value was 4.4 mg/I.

Results with reference substance (positive control):

The quality of the Daphnia will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l).

Last reference control at January 25, 1995 EC50 : 0.95 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 (issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/L, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.

During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 1.04 mg/l, the EC100 value was 4.4 mg/I.

Executive summary:

The acute toxicity of the test item to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 (issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992).

The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

The nominal concentrations tested were 0.40 / 0.88 / 1.9 and 4.3 mg test article/L, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1 g/1 stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours.

During the test the test article concentrations in the analysed test media were in the range of 102 - 120 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test.

No immobilization and abnormal behaviour of the Daphnia was observed in the controls. The NOEC and EC0 value after 48 hours were determined to be 1.04 mg/l, the EC100 value was 4.4 mg/I.