Tetrakis(1-piperidyl)phosphonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

November 15th, 2005 - November 17th, 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Test material form:

solid

Specific details on test material used for the study:

Purity: 96%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution: The solution (100 mg/L test was weighed out) was prepared with dilution water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS (Clone 5)
Origin: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu)
Breeder: Dr.U.Noack-Laboratorium, 31157 Sarstedt, Germany
Age of test organisms: 2 - 24 h
Culture: In 2-3 L glass vessels with approx. 1.8 L culture medium, at 21°C (temperatures of 20 - 25°C are tolerated) in an incubator, 16 h il
lumination, illumination strength max 20µE/(m²s).
Culture medium: ELENDT M4, modified to a total hardness of 160 - 180mg CaCO3/L.
Feeding: At least 5 times per week ad libitum with a mix of Desmodesmus subspicatus and Chlorella
vulgaris.

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Dilution water according to EC 92/69 L383A C.2.: KCl 5.76 mg/L, NaHCO3 64.8 mg/L, CaCl2*2 H2O 294 mg/L, MgSO4*7 H2O 123 mg/L

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Dilution water: 262 mg CaCO3/L

Test temperature:

18-22°C

pH:

Test: 7.22 - 7.98 (48h)

Dissolved oxygen:

Test: 7.73 - 7.99 mg/L (48h)

Nominal and measured concentrations:

Nominal: 0.256, 0.64, 1.60, 4.00 and 10.0 mg/L
Mean measured: 0.20, 0.54, 1.37, 3.56 and 8.96 mg/L

Details on test conditions:

20 test organisms were exposed to each test concentration and control.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

6.27 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

2.91 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

8.45

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Confidence interval p = 95% [mg/L]

Remarks:

7.63 - 9.35

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.88

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Confidence interval p = 95% [mg/L]

Remarks:

3.52 - 4.28

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 8.96 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 6

Duration:

48

Dose descriptor:

EC100

Effect conc.:

8.96

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 7

Duration:

48

Dose descriptor:

NOEC

Effect conc.:

1.37 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 8

Duration:

48 h

Dose descriptor:

LOEC

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The EC10-values and EC50-values after 24 and 48 hours were calculated by probit analysis.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50(48h) of Tetrakis(piperidino)phosphonium chloride on Daphnia Magna (immobilisation) was determined at 3.88 mg/L.