[2-[[2-cyano-3-[4-[ethylbenzylamino]phenyl]-1-oxoallyl]oxy]ethyl]trimethylammonium chloride

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 March 2017 to 19 April 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Remarks:

urban (about 66%) and industrial (about 34%) liquid sewage

Details on inoculum:

- Source of inoculum/activated sludge: urban (about 66%) and industrial (about 34%) liquid sewage situated at San Rocco - Monza (MB), Italia.
- Storage conditions: under aerobic conditions
- Storage length: no data
- Preparation of inoculum for exposure: centrifuged and washed
- Pretreatment: none
- Concentration of sludge: 4 mg/L SS

Duration of test (contact time):

28 d

Initial conc.:

15.08 mg/L

Based on:

TOC

Remarks:

determined by REDOX SnC using CHN analyser

Parameter followed for biodegradation estimation:

inorg. C analysis

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD 310
- Test temperature: 20°C
- pH: not indicated
- pH adjusted: no
- Suspended solids concentration: 4 mg/L
- Continuous darkness: yes
- Other: contimuous stirring
TEST SYSTEM
- Culturing apparatus: 1000 mL volumteric flask
- Number of culture flasks: 3/sampling and 5 for the sampling after 28 days for test substance, blancs and reference substance
- Measuring equipment: TOC analyser
- Test performed in closed vessels: yes

SAMPLING
- Sampling frequency: on day 1, 11, 14, 21 and 28 in blanks, reference substance controls and test substance treated bottles (3 replicates per sampling time except at day 28 5 replicates)
- Sampling method: in individual bottles reaction stopped with the addition of 6 mL of sodium hydroxide (1 M) (12 h before measuring)
- Sample storage before analysis: none

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes


STATISTICAL METHODS:

Reference substance:

benzoic acid, sodium salt

Remarks:

at 20.01 mg/L as C

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

13

Sampling time:

1 d

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

51

Sampling time:

11 d

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

55

Sampling time:

14 d

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

64

Sampling time:

21 d

Key result

Parameter:

% degradation (inorg. C analysis)

Value:

75

Sampling time:

28 d

Details on results:

The substance is biodegradable, but does not meet the 10-day window criterion

Results with reference substance:

see table

CHECK POINT (Days)	% OF BIODEGRADATION Reference substance	% OF BIODEGRADATION Test item
0	0	0
1	27	13
11	87	51
14	95	55
21	99	64
28	100	75

 

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

the substance is considered to be readily biodegradable, but failing 10-day window.

Executive summary:

In a test according to OECD 310, the substance (15.08 mg C/L) degraded 75% over a period of 28 days. The substance failed the 10 -day window.

Description of key information

In a test according to OECD 310, the substance (15.08 mg C/L) degraded 75% over a period of 28 days. The substance failed the 10 -day window.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable but failing 10-day window

Type of water:

freshwater