Benzeneacetic acid, .alpha.-hydroxy-, ammonium salt (1:1), (.alpha.R)-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000-12-18

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzeneacetic acid, .alpha.-hydroxy-, monoammonium salt, (.alpha.R)-

EC Number:

606-826-3

Cas Number:

217308-07-1

Molecular formula:

C8 H8 O3 .H3 N

IUPAC Name:

Benzeneacetic acid, .alpha.-hydroxy-, monoammonium salt, (.alpha.R)-

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): (R)-Ammoniummandelat solution (ca. 30 %), purity
- Physical state: liquid, light yellowish, Iight turbid
- Analytical purity: 29.1-31.2 % Ammoniummandelat, 70 % water
- Lot/batch No.: 30193/89
- Expiration date of the lot/batch: no limitation of storage
- Storage condition of test material: storage at the refrigerator (4-10°C)
- Water-solubility: miscible in any relation

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Stock solution: 2000mg of the test substance was added to 1000 ml deionised water.
- Added volume of stock solution: 125.0 mI/vessel

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Inoculum: Activated sludge from laboratory wastewater plant treating municipal sewage
- Preparation of inoculum: washed, conc. of dry matter: 5 g/l, aerated during the night

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Test conditions

Test temperature:

20 +- 2 °C

pH:

6.8-7.2

Nominal and measured concentrations:

nominal: 0, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flask (250 ml)
- Material, size, headspace, fill volume: 250 ml
- Oxygen concentration during aeration: > 2.5 mg/l
- Oxygen concentration immediatley before measurement: > 6.5 mg/l
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
- Biomass loading rate: inoculum: 50 ml (final conc. of dry matter: 1 g/l)


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml (100-fold concentrated OECD medium)
- Dilution water: deionised water

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Duration of measurement of oxygen consumption: 8-10 min

TEST CONCENTRATIONS
- Test concentrations: 1000 mg/L

Reference substance (positive control):

yes

Remarks:

3.5 dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC50: ca. 5.5 mg/l (30min)

Any other information on results incl. tables

parameter            BC (average)   TS      RS1   RS2   RS3
oxygen concentration (mg/l)
   start             7.7-8.3        7.9     8.5   9.4   8.8
   6 min             5.2-5.8        5.5     6.3   8.5   8.7

oxygen consumption rate
(mg O2/l/h)          25             24      23     9     1

specific oxygen consumption rate
(mg O2/l/h)          25             24      23     9     1

change in oxygen consumption rate compared to BC
(%)                   -              4       8    64    96

BC: blank control

RS: reference substance (conc.: RS1: 1 mg/l; RS2: 10 mg/L; RS3: 100 mg/L)

TS: test substance 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes