4-hydroxy-2-methylnaphthyl benzoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to OECD and GLP guidelines

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-hydroxy-2-methylnaphthyl benzoate

EC Number:

218-647-4

EC Name:

4-hydroxy-2-methylnaphthyl benzoate

Cas Number:

2211-28-1

Molecular formula:

C18H14O3

IUPAC Name:

4-hydroxy-2-methyl-1-naphthyl benzoate

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

-

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

the calculated LC50 of Monobenzoat after 96 h was 1.3 mg/l with a 95 % confidence limit of 1.0 - 1.7
mg/l.

Executive summary:

A static test procedure was used to assess the acute toxicity of the test substance Monobenzoat to Rainbow Trout, oncorhynchus mykiss. The fish were exposed for 96 hours to aqueous solutions of the test substance at nominal test concentrations of 10.0, 5.6, 3.1, 1.7, and 1.0 mg/l in the presence of TWEEN 80 as an emulsifier. Additional groups of fish were exposed to dilution water (blank) and to the highest amount of solvent used in the test (solvent control). Observations regarding mortality and any visible abnormalities were made after 2, 4, 6, 24, 30, 48, 54, 72, 78 and 96 hours. Temperature, pH, and oxygen concentration were measured in the test solutions at the beginning of the study and at 24-hour intervals thereafter throughout the study. The amount of dissolved test substance in the presence of TWEEN 80 in the highest test concentration was 0.9 mg/l at the beginning of the test. There was no mortality in the blank and in the solvent control. The maximum concentration causing no mortality within the test period after 96 h (LC0) was 1.0 mg/l. The minimum concentration causing 100 per cent mortality after 96 h (LC100) was 1.7 mg/l. The No Observed Effect Concentration after 96 h (NOEC) was 1.0 mg/l. All these values are based on nominal concentrations. The calculated LC50 of Monobenzoat after 96 h was 1.3 mg/l with a 95 % confidence limit of 1.0 - 1.7 mg/l.