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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No GLP and no analytical monitoring.
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
duration only 24 hours, no analytics
Principles of method if other than guideline:
test duration 24h, no analytical verification
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Stock solution prepared 10000 mg/l.
Dilutions were made from stock.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain: Daphnia magna straus
- Source: Institut National de Recherche Chimique Appliquee, France
- Age at study initiation: 6-24h
- Feeding during test: no
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
2.55 mmol/l
Test temperature:
292-294 K
pH:
8.1
Nominal and measured concentrations:
905, 1280, 1810, 2560, 3620, 5120, 7240, 10239 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4

OTHER TEST CONDITIONS
- Photoperiod:16h
- Light intensity: 5 Mikroeinstein (400-750 nm)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilized daphnids at 0 and 24 h
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
8 609 mg/L
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
7 240 mg/L
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
10 239 mg/L
Basis for effect:
mobility
Conclusions:
Sodium nitrate did not induce acute immobilisation of Daphnia magna up to 7240 mg/l after 24 hours of exposure (EC 0). EC50 was 8609 mg/l after 24 h.

Description of key information

A 24h EC50 of 8600 mg/L  has been derived for sodium nitrate in a reliable OECD 202 guideline study with daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
8 600 mg/L

Additional information

Several studies with invertebrates are present with sodium nitrate, all showing very high EC50s. In a reliable OECD 202 guideline study with daphnia magna, the 24 hr EC50 was determined to be 8600 mg/L. In another study a 48 hr EC50 was derived to be 3581 mg/L, although limited information on materials and methods was available, but supporting the 24 hr EC50. Studies with other invertebrates showed results expressed as NO3 -, EC50s being >100 or >1000 mg/L.