Ethylene di(acetate)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 Jul - 19 Jul 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 120 mg/L (nominal)
- Sampling method: Aliquots of the test substance (1.4 mL) and the control exposures were collected from approximately mid-depth of the chromatography jars and combined with a nominal 0.14 mL of internal standard. At test end (time 48-h), aliquots were collected directly from the test vessels and submitted for concentration verification.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared in dilution water at nominal 24 mg/mL. Appropriate volume was added to give a final nominal concentration of 120 mg/L. After addition, the exposure solutions were stirred with a Teflon coated stir rod and an aliquot of 200 mL was added to the testing vessels.
- Eluate: no
- Differential loading: no
- Controls: water control

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation: < 24 h
- Method of breeding: Daphnids were reared within 100 L culturing tanks. Gravid daphnids used to produce the test animals were obtained from the rearing tanks which had been established for at least two weeks. Prior to the study, approx. 100 gravid daphnids were transferred by net into two glass bowls containing dilution water and food. After approx. 24 h the adults were removed and the neonates were used for testing.
- Feeding during test: none

ACCLIMATION
- Acclimation period: two weeks
- Acclimation conditions: same as test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

120 mg CaCO3/mL

Test temperature:

20 - 21 °C

pH:

7.7 - 8.4

Dissolved oxygen:

7.6 - 9.1 mg/L

Nominal and measured concentrations:

Nominal: 0, 120 mg/L
Measured: 0, 116.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Type: Covered with clear watch glasses and sealed with Parafilm.
- Material, size, headspace, fill volume: glass, 300 mL, fill volume: 200 mL, headspace: 100 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Pumped from a large underground storage reservoir and passed through polypropylene filter tubes, through a series of activated carbon filter tubes and finally through another set of polypropylene filter tubes. The filtered water stream was then treated with sodium thiosulfate via a chemical injection system. The filtered-treated water was then tempered to 20 ± 2 °C by passage through a heat-exchange unit and distributed throughout the testing facility through stainless steel and PVC piping.
- Total organic carbon: 3.2 mg/L
- Metals: < detection limit
- Pesticides: < detection limit
- Chloride: 23.7 mg/L
- Alkalinity: 92.4 mg CaCO3/mL
- Volatile organics: < detection limit
- PCBs: < detection limit
- Conductivity: 290 µmhos/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark (30 min transition period)

EFFECT PARAMETERS MEASURED: Mobility and behaviour at 0, 24 and 48 h.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 116.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Mean from two replicates

Details on results:

- Behavioural abnormalities: No behavioural abnormalities were observed in the control and test substance exposure solutions.
- Mortality of control: no

One daphnia was immobile after 24 h in one replicate at a test substance concentration of 120 mg/L (nominal).

 Table 1: Analyzed concentrations and % loss of test substance during this study.

Nominal concentration [mg/L	Concentrations of analyzed concentrations [mg/L]				% Loss
	Replicate	0 h	48 h	geometric mean
control	A	ND	ND	NA	NA
control	B	ND	ND	NA	NA
120	A	114.69	112.19	113.43	2.2%
120	B	124.22	114.14	119.07	8.1%

ND: Not detected

NA: Not applicable

Description of key information

EC50 (48 h): > 116.3 mg/L for Daphnia magna (OECD 202)

Additional information

One experimental study investigating the short-term toxicity of ethylene diacetate (CAS 111-55-7) to aquatic invertebrates is available (Hirsch, 2000). The study was performed according to OECD 202 (GLP) with the water flea Daphnia magna. One test concentration of 120 mg/L (nominal, limit test) was tested in a static exposure regime. The nominal concentration was measured by GC/FID analysis. No effects on the mobility of D. magna were observed resulting in an EC50 (48 h) > 116.3 mg/L (meas. geom. mean). Deviations between nominal and measured concentrations were < 10% throughout the test.