Benzyl benzoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15/3/1993 - 19/3/1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

Temperature decrease greater than 1oC

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Due to the semi-static nature of this test concentrations were measured in new test solutions and in used test solutions when they were being replaced (every 24 hours).

Vehicle:

no

Details on test solutions:

Test solutions were created by dissolving a directly weighed amount of the test substance in water using an ultra-turrax. Nominal test concentrations were 0 (control), 3.9, 5.5, 7.8 and 11 mg/l.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Species: Zebrafish (Brachydanio rerio)
Origin: West-aquarium (Bad Lauterberg)
Date of birth: 12/09/1992
Date of delivery: 26/11/1992
Body length: 2.5-3.5 cm
Pre-test holding: synthethic freshwater according to ISO

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

14.2 dH

Test temperature:

21.4-22.7

pH:

7.2-8.0

Dissolved oxygen:

7.8-9.7 mg/l

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 0 (control), 3.9, 5.5, 7.8, 11 mg/l

Details on test conditions:

See methods below

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.32 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.9 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.84 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. Following discussion with the German authority for environmental affairs (Umweltbundesamt) the test results were based on the arithmetic mean from the beginning and end of all exposure times. Based on this the LC0/LC100 was calculated to be 2.32 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 2.32 mg/l.

Results with reference substance (positive control):

No information provided

Reported statistics and error estimates:

No information provided

Sublethal observations / clinical signs:

Table 1: Cumulative mortality (%) of fish at all test concentrations and observation points.

Test concentration (mg/l)	Cumulative mortality (%) 2 hours	Cumulative mortality (%)24 hours	Cumulative mortality (%) 48 hours	Cumulative mortality (%)72 hours	Cumulative mortality (%)96 hours
0 (control)	0	0	0	0	0
3.9	0	0	0	0	0
5.5	0	30	60	100
7.8	0	90	100
11	0	100

Validity criteria fulfilled:

not specified

Conclusions:

The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 0.29 mg/l.

Executive summary:

A 96 hour acute fish toxicity study was carried out in accordance with EU method C1. Brachydanio rerio were exposed to the test substance at concentrations of 0 (control), 3.9, 5.5, 7.8 and 11 mg/l. A semi-static regime was used and test media was changed every 24 hours. Temperature, pH and dissolved oxygen concentration was measured in new and used media. The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. Following discussion with the German authority for environmental affairs (Umweltbundesamt) the test results were based on the arithmetic mean from the beginning and end of all exposure times. Based on this the LC0/LC100 was calculated to be 2.32 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 2.32 mg/l.

Description of key information

In a 96-hour acute toxicity study the LC50 of benzyl benzoate to Brachydanio rerio was determined to be 2.32 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

2.32 mg/L

Additional information

A 96 hour acute fish toxicity study was carried out in accordance with EU method C1.Brachydanio rerio were exposed to the test substance at concentrations of 0 (control), 3.9, 5.5, 7.8 and 11 mg/l. A semi-static regime was used and test media was changed every 24 hours. Temperature, pH and dissolved oxygen concentration was measured in new and used media. The 96-hour geometric mean of the LC0/LC100 was found to be 0.29 mg/l. Following discussion with the German authority for environmental affairs (Umweltbundesamt) the test results were based on the arithmetic mean from the beginning and end of all exposure times. Based on this the LC0/LC100 was calculated to be 2.32 mg/l. This is considered to be equivalent to the LC50. As such the LC50 can be considered to be 2.32 mg/l.