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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientifically acceptable guideline study, Non-GLP. Inhibition control is used for the assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Test procedures according to EU Directive 79/831/EEC Annex V, part C: Methods for the determination of the ecotoxicity degradation - biodegradation, Manometric respirometry test. This method is comparable to the OECD guideline 301 F.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Source of inoculum/activated sludge: Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage. Concentration of dry substance 30 mg/I.
- Laboratory culture: yes

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
28 d

Test conditions

Test temperature:
room temperature
pH:
6.6 - 7.4
Nominal and measured concentrations:
- Other: nominal concentrations from 45-180 mg/l test substance, 32-127 mg/l DOC
- inhibition control : 90 mg/L test substance
Details on test conditions:

TEST SYSTEM
- Culturing apparatus: glass bottles, closed system
- Number of culture flasks/concentration: 2-3
- Method used to create aerobic conditions: on a magnetic stirrer, aerated with CO2-free air

SAMPLING
- Sampling frequency: daily

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance (positive control):
yes
Remarks:
Anilin

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
90 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: inhibition control of readily biodegradability test

Applicant's summary and conclusion