1-Propene, hydroformylation products, high-boiling

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Oxooel 740
- Physical state: Liquid, yellowish, turbid
- Analytical purity: 2-Ethylhexane-1,3-diol: 44 area-%
- Batch No.: continuous production
- Storage condition of test material: at ambient temperatures under N2

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solutions were prepared separately for each test concentration. The solutions were stirred for approx. 30 min at 20 ± 2 °C
- Differential loading: yes

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: green alga
- Strain: SAG 61.81
- Source (laboratory, culture collection): Collection of algal cultures in University of Göttingen/Germany
- Method of cultivation: A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 4 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22 °C

pH:

Uninoculated samples (0 h): 7.2
Inoculated samples (0 h): 7.2
Unioculated samples (72 h): 7.8 - 7.9
Inoculated samples (72 h): 7.9 - 10.3

Nominal and measured concentrations:

Nominal test concentrations: control, 4.6, 10, 22, 46, 100 mg/L
Mean measured concentrations: -, 3.2, 8.1, 18, 40.5, 91.6 mg/L
Recovery rates: -, 70, 81, 82, 88, 92%

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, nominal volume, fill volume: Erlenmeyer flasks with glass plugs, 250 ml, 300 ml (flasks completely filled)
- Initial cells density: 0.3 x 10E4 cells/ml
- No. of vessels per concentration (replicates): 3 inoculated vessels and 1 uninoculated vessel
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium prepared according to OECD Guideline for Testing of Chemicals
- Intervals of water quality measurement: pH after 0 and 72 hours, temperature continuously

OTHER TEST CONDITIONS
- Photoperiod: Artificial light, type universal white (OSRAM L 25), permanent illumination
- Light intensity and quality: Approx. 5459 Lux at a wave length of 400 - 700 nm

EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: After the end of the exposure the control replicates were mixed and serially diluted by factor 2. The fluorescence in the dilutions and the undiluted mixture were measured by single measurements and in parallel by direct microscopic cellcounting (two counts in a Neubauer haemocytometer).
- Chlorophyll measurement: In vivo chlorophyll-a fluorescence (pulsed excitation with light flashes having a wavelength of 432 nm)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: yes
- Results used to determine the conditions for the definitive study: ErC50 = 99 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

51 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

98 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

40.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

43.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

65.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC90

Effect conc.:

96.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Effect concentrations 8

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: yield

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): low cell density at 100 mg/L

Measured effect concentrations after 72 hours based on growth rate:
EC10 = 45.2 mg/L
EC50 = 89.6 mg/L
EC90 > 91.6 mg/L
NOEC = 40.5 mg/L
Measured effect concentrations after 72 hours based on yield:
EC10 = 38.5 mg/L
EC50 = 58.7 mg/L
EC90 = 88 mg/L
NOEC = 18 mg/L

Results with reference substance (positive control):

ErC50 (72 h): 0.92 mg/L (Control experiment: 06 Oct 2008)