1-Propene, hydroformylation products, high-boiling

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study incl. analytics

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Oxooel 740
- Physical state: Liquid/ yellowish, clear
- Analytical purity: Sum of identified hydroformylation products: 85.4 cor.-area-% several other components could be quantified (see report 09L00241 chapter 3): 13.5 cor.-area-% Sum of identified compounds: 98.9 cor.-area-%
- Lot/batch No.: TK285_20090716
- Expiration date of the lot/batch: 2010-07-16
-Water solubility: poorly soluble (information from sponsor). Soluble up to 100 mg/L in test media (observation of Ecotoxicology Lab)
- Stability under test conditions: stable under storage conditions over the exposure period.
- Storage condition of test material: The test substance was stored in a refrigerator, dry storage.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 0.32, 1.0, 3.2, 10, 32 mg/L as nominal concentrations based on test substance mass without correction for purity
- Samples were taken on the following days:
Interval / Week 1
Day 0 (fresh solutions)
Day 2 (fresh and 48 hour old test solution)
Day 5 (fresh and 72 hour old test solution)
Day 7 (48 hour old test solution)
Interval / Week 2
Day 12 (fresh solutions)
Day 14 (48 hours old test solutions)
Interval / Week 3
Day 19 (fresh solutions)
Day 21 (48 hours old test solutions)
- Sample storage conditions before analysis: Sample transport to the Analytical Laboratory was done on the day of sampling. Storage at room temperature. The retained samples were stored in a refrigerator until sent to the analytical laboratory.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test item was homogenized by shaking the container before use and all test solutions were prepared individually (differential loading). The test substance is weighed, placed in a volumetric flask and filled to the mark with test medium and closed with a glass stopper. The solution was stirred for approximately 30 min at 20 ± 2 °C and visually checked for any undissolved test substance. All stock solution appeared colorless-clear

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978.
From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxic
- Body length of parent animals: 4.4 mm before exposure
- Feeding during test: During the test daphnids were fed daily a diet of live green algae Desmodesmus subspicatus, cultured in a synthetic medium. The algae were separated from their culture medium by centrifugation, resuspended in daphnid's medium (M4) corresponding to concentrations of 1110 mg and 1160 mg TOC*/L (respectively) in the algal concentrates used. The daphnids were fed a defined volume (≤180 μL) of the concentrate to reach the amount of food defined in the table below. The algae were stored in a refrigerator (dark, about 4-8°C) for maximum 21 days.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

2.46-2.52 mmol/L

Test temperature:

20°C

pH:

8.0-8.6

Dissolved oxygen:

8.4-9.2

Nominal and measured concentrations:

Concentrations: 0, 0.32, 1.0, 3.2, 10, 32 mg/L as nominal concentrations based on test substance mass without correction for purity

Details on test conditions:

TEST SYSTEM
- Test vessel: Numbered glass beakers (nominal volume 50 mL), sealed with ground glass stoppers. The test vessels were sealed and completely filled due to the volatility of the test substance.
- Material, size, headspace, fill volume: approx. 50 mL
- Type (delete if not applicable): closed
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10 (10 replicates)
- No. of vessels per control (replicates): 10 (10 replicates)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) is used as media for culture and test purposes. For the composition of this M4 medium see OECD 211.
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: pH value: 7.5 – 8.5
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: About 631 - 706 lux at a wave length of 400 -750 nm
(recommended values in lux units provided in ISO 10706)
- Exposure conditions: Semi-static exposure via test water

VEHICLE CONTROL PERFORMED: no

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of parent animals: 30% in the highest test group (32 mg/L) after 21 days of exposure
- Reproduction: 46% in the highest test group (32 mg/L) after 21 days of exposure
- Body length of parent animals: 4.4 mm before exposure, 4.2 mm in the highest test group (32 mg/L) after 21 days of exposure

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A significant effect on parent mortality and reduced reproductive output was observed in the highest test group (32 mg/L) after 21 days of exposure. In addition a significant effect on parent growth (as length) was also observed in the highest test group. As effects were only observed in the highest test group, ECx point estimates could not be reliably calculated.