Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Type of information:
- calculation (if not (Q)SAR)
- Adequacy of study:
- supporting study
- Study period:
- 2014-06-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Alignment of protein sequences from protein eADF4(C16) with the UniProt Knowledgebase (UniProtKB) database of protein sequences.
- GLP compliance:
- no
- Type of method:
- other: in silico
- Endpoint addressed:
- other: biological activity
Test material
- Reference substance name:
- Recombinant spider silk protein eADF4(C16) derived from E. Coli by fermentation
- Molecular formula:
- not applicable, UVCB
- IUPAC Name:
- Recombinant spider silk protein eADF4(C16) derived from E. Coli by fermentation
- Test material form:
- other: protein film on a cellulose matrix
Constituent 1
Results and discussion
Applicant's summary and conclusion
- Conclusions:
- Over 10.000 peptide sequences of the UniProt Knowledge Base (as of June 2013)
were analysed in respect to known peptide annotations. The resulting peptide fragments were
submitted to a sequence alignment with eADF4(C16) via an exact string-matching method. None
of the sequences aligned with the silk protein, indicating to no biologic activity of eADF4(C16) - Executive summary:
None of the peptide sequences annotated in UniprotKB could be found in exact match in the protein sequences eADF4(C16). All control sequences were found in eADF4(C16) by the exact string matching procedure.
After performing the alignment as described above, there are no matches - neither on the total sequences eADF4(C16) or partial sequences - and thus no evidence for biological activity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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