Amines, C12-14-alkyl

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 106 (Adsorption - Desorption Using a Batch Equilibrium Method)

GLP compliance:

yes (incl. QA statement)

Type of method:

batch equilibrium method

Media:

other: soil sediment and sludge

Test material

Test material information

Constituent 1

Reference substance name:

Octadecylamine

EC Number:

204-695-3

EC Name:

Octadecylamine

Cas Number:

124-30-1

IUPAC Name:

octadecan-1-amine

Details on test material:

Test substance: Octadecylamine, 14C-labelled (NOTOX test substance 98487)

Radiolabelling:

yes

Study design

Test temperature:

20°C ± 2°C

Batch equilibrium or other method

Analytical monitoring:

yes

Details on matrix:

SOIL 1: SSLRC Standard soil No 164 (Cranfield 164)
- Geographic location: Farditch Farm, Buxton, Derbyshire, United Kingdom. OS map reference
SK104691
PROPERTIES
- Soil texture:
- Particle size distribution(USDA):
% silt (2-53 μm): 63.24
% sand (53-2000 μm): 15.00
% clay (< 2 μm): 20.04

- Particle size distribution (DIN)
% silt (2-63 μm): 70.08
% sand (63-2000 μm): 9.87
% clay (< 2 μm): 20.04
- Horizon: 10-20 cm
- pH: 6.3-6.8
- Organic carbon (%): 3.8
- CEC (meq/100 g): 21.8

SOIL 2: SSLRC Standard soil No 266 (Cranfield 266)
- Geographic location: Posher Farm, Ufton, Warwicks, United Kingdom. OS map reference SP
37076231
PROPERTIES
- Soil texture:
- Particle size distribution(USDA):
% clay (< 2 μm) 50.17
% silt (2-53 μm) 29.00
% sand (53-2000 μm) 20.84

- Particle size distribution (DIN)
% clay (< 2 μm) 51.63
% silt (2-63 μm) 29.77
% sand (63-2000 μm) 18.60
- Horizon: 0-20 cm
- pH: 7.4-8.6
- Organic carbon (%): 1.5
- CEC (meq/100 g): 23.1

SEDIMENT:
- Details on collection: The sediment was sampled from Oostvaardersplassen (OVP) on May 10, 2000. Oostvaardersplassen
is an area endiked from the lake IJssel (IJsselmeer), situated near Lelystad, The Netherlands. The lake
has been closed for river Rhine water for approximately 35 years.
- Textural classification (i.e. %sand/silt/clay): silt loam (% clay (< 2 μm): 18.70, % silt (2 μm – 63 μm): 69.02, % sand (63 μm – 2 mm): 12.28)
- pH: 7.6 - 8.0
- Organic carbon (%): 2.4
- CEC (meq/100 g): 13.6

SEWAGE SLUDGE:
- Type of sludge:
- Source of sludge: The sewage sludge was sampled from the municipal wastewater treatment plant Waterschap De
Maaskant in ’s-Hertogenbosch, The Netherlands on May 15, 2000 from a basin containing so-called
return sludge, which had been thickened two to three times
- Oxygen status:
Texture: Silty Clay
% clay (< 2 μm): 45.93
% silt (2 μm – 63 μm): 51.57
% sand (63 μm – 2 mm): 2.50
% organic carbon: 30.1
% organic matter: 51.9
pH-H2O: 7.2
pH-CaCl2: 6.8
CEC (meq/100g): 54.9

Details on test conditions:

TEST CONDITIONS
- other: Prior to the start of all adsorption-desorption experiments, a precisely known amount of approximately
100 mg of soil or sediment or approximately 1000 mg of sludge was weighed out in glass vials and a
known amount of approximately 10 mL (soil and sediment) or 9 mL (sludge) of 0.01 M aqueous CaCl2
solution was added. The vials were sealed and placed on a shaker for at least 16 hours for preequilibration.
Soils were equilibrated at 20°C ± 2°C.

Results and discussion

Any other information on results incl. tables

Octadecylamine can be considered immobile in Cranfield 164 silt loam soil, Cranfield 266 clay soil, Oostvaardersplassen sediment silt loam and sewage sludge DB1 silty clay.

Table 1: K (F, o,)ads values, optained from Freundlich adsorption isotherm, were:

Test system	K (F, om) ads [µg^1 -1/n(cm³)^1/n g^-1]	1/n
Cranfield 164	46437	1.5384
Cranfield 266	1155884	1.8897
Sediment OVP	155466	1.4478
Sludge DB 1	1583	1.0322

for all test systems r² > 0.96

Table 2: Desorption kinetics:

Test system	% desorption after 47 hours	% desorped after 166 hours
Cranfield 164	24.4	24.2
Cranfield 266	13.7	19.1
Sediment OVP	No result	16.6
Sludge DB 1	5.7	No result

The result clearly show that octadecylamine desorption occurs to a lesser extent than adsorption and is slower than adsorption for all test systems.

Applicant's summary and conclusion

Executive summary:

The adsorption behaviour of octadecylamine in soils, sediment and sludge was studied in two soils, one sediment and one sewage sludge: Cranfield 164 soil [21.8% clay, 6.6% organic matter, silt loam], Cranfield 266 soil [50.2% clay, 2.6% organic matter, clay], Oostvaardersplassen sediment [18.7% clay, 4.1% organic matter, silt loam] and sewage sludge DB1 [45.9% clay, 51.9 % organic matter, silty clay]. These test systems encompass a range of % clay and % organic material. Because of the strong adsorption of octadecylamine on the container materials, an alternative set-up for performance of the study was developed. In this method, activity was determined in the supernatant and on the container walls. Activity adsorbed to the test system was calculated from these results and the initial activity applied. Adsorption and desorption kinetics were determined at an initial concentration of approximately 1 μg/mL. Adsorption isotherms were determined over a concentration range from approximately 0.04 to 5 μg/mL. All adsorption-desorption experiments were carried out at 20°C ± 2°C. Mass balances thus obtained for the adsorption isotherm experiment were all > 90%, indicating the validity of the test set-up. Octadecylamine can be considered immobile in Cranfield 164 silt loam soil, Cranfield 266 clay soil, Oostvaardersplassen sediment silt loam and sewage sludge DB1 silty clay. KF,o m ads values, obtained from the Freundlich adsorption isotherm, were 46*103 μg1-1/n(cm3)1/ng-1 (Cranfield 164 silt loam, 1/n = 1.5384), 1.2*106 μg1-1/n(cm3)1/ng-1 (Cranfield 266 clay, 1/n = 1.8897), 1.6*105 μg1-1/n(cm3)1/ng-1 (Oostvaardersplassen sediment silt loam, 1/n = 1.4478) and 1.6*103 μg1-1/n(cm3)1/ng-1 (sewage sludge DB1 silty clay, 1/n = 1.0322). For all test systems r2 > 0.96.