Amines, C12-14-alkyl

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water: sewage treatment simulation testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: ISO 11733 (1995)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Amines, coco alkyl

EC Number:

262-977-1

EC Name:

Amines, coco alkyl

Cas Number:

61788-46-3

IUPAC Name:

61788-46-3

Details on test material:

- Name of test material (as cited in study report):Cocoamine

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: activated sludge and wastewater was collected from the treatment plant of Nieuwgraaf in Duiven, Netherlands.
- Concentration of sludge: 300 ml of activated sludge containing approximately 3 g/L dry weight was used as an inoculum for each CAS unit.

Duration of test (contact time):

23 d

Initial test substance concentration

Initial conc.:

57 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

test mat. analysis

Details on study design:

TEST CONDITIONS
- Test temperature: 20 -22 °C
- pH: 6.7 - 7.2

TEST SYSTEM
- Culturing apparatus: A reactor with an aeration compartment and seperator was used (non-coupled)
- Number of culture units: 2

Reference substance

Reference substance:

other: Potassium hydrogen phthalate

Results and discussion

Any other information on results incl. tables

Table 1: Removal of cocoamine in the control and test unit

Day	Control	Test
	Removal (%)
17	*	>99.98
19	*	>99.97
21	*	>99.98
22	*	>99.97
23	*	>99.98
Mean	*	>99.98

* not calculated because many measurements were below the detection limit.

The high removal percentage demonstrates that the continuous activated sludge system treating domestic wastewater spiked with cocoamine performs very well. These results strongly indicate that cocoamine is completely (ultimately) degradad by microorganisms. Finally the high removal percentages demonstrate that recalcitrant water-soluble substances are not formed during the biodegradation test.

Table 2: Alkylamine Concentrations measured in the influent and effluent of the control and test unit and the calculated cocoamine concentration. the cocoamine concentartion was calculated by adding up the concetration of the alkylamines (C12 -18) and unsaturated octadecylamine (C18´).

Sample	C12 amine	C14 amine	C16 amine	C18 amine	C18´ amine	C12-18 amine
	µg/L
Influent control	6.6	2.2	1.3	<1.0	<3.0	<14.1
Effluent control (day 17)	<1.0	<1.0	<1.0	<1.0	<3.0	<7.0
Effluent control (day 19)	<1.0	<1.0	<1.0	<1.0	<3.0	<7.0
Effluent control (day 21)	<1.0	<1.0	<1.0	<1.0	<3.0	<7.0
Effluent control (day 22)	<1.0	<1.0	<1.0	<1.0	<3.0	<7.0
Effluent control (day 23)	<1.0	<1.0	<1.0	<1.0	<3.0	<7.0
Influent test	26000	7500	330	88	16	33934
Effluent test (day 17)	1.8	<1.0	<1.0	<1.0	<3.0	<7.8
Effluent test (day 19)	<1.0	<1.0	<1.0	2.7	<3.0	<8.7
Effluent test (day 21)	<1.0	<1.0	<1.0	<1.0	<3.0	<7
Effluent test (day 22)	<1.0	<1.0	<1.0	1.5	3.8	<10.3
Effluent test (day 23)	<1.0	<1.0	<1.0	<1.0	<3.0	<7

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