Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 482-160-5
CAS number: 130786-09-3
The study was performed according the requirements
of OECD TG 474, EU Method B.12 and US EPA OPPTS 870.5395 and Japan
MHLW/METI guidelines under GLP conditions. Within the study a
preliminary range-finding test was performed to find suitable dose
levels of the test item, route of administration and to investigate to
see if there was a marked difference in toxic response between the sexes
at 2000 mg/kg and 1000 mg/kg dose levels. There was no marked difference
in toxicity of the test item between the sexes; therefore the main test
was performed using only male mice. The micronucleus test was conducted
using the oral route in groups of seven mice (males) at the maximum
tolerated dose (MTD) 1000 mg/kg and with 500 and 250 mg/kg. Exposure was
via oral gavage and then terminations were completed at 24 or 48 hours,
as applicable. The femoral bone marrow was extracted, and smear
preparations made and stained. Polychromatic (PCE) and normochromatic
(NCE) erythrocytes were scored for the presence of micronuclei. Within
the vehicle controls further groups of seven males were dosed with the
vehicle (arachis oil) and then terminations were completed at 24 or 48
hours, as applicable. A positive control group utilising
cyclophosphamide at 50 mg/kg was also employed and terminated at 24
hours. There were no premature deaths seen in any of the dose groups.
Clinical signs were observed in animals dosed with the test item at and
above 250 mg/kg in both the 24 and 48 hour groups where applicable,
these were as follows: Hunched posture, ptosis, ataxia, lethargy and
splayed gait. No statistically significant decreases in the PCE/NCE
ratio were observed in the 24 or 48 hour test item dose groups when
compared to their concurrent control groups. However, the observation of
clinical signs was considered to indicate that systemic absorption had
occurred. No statistically significant responses were observed in the
test item treatment groups up to 1000 mg/kg bw relative to the
concurrent vehicle control. The positive control group showed .a marked
increase in the incidence of micronucleated polychromatic erythrocytes
hence confirming the sensitivity of the system. Under the conditions of
this study, the test item would not be considered as genotoxic.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Sellel veebilehel kasutatakse küpsiseid, et tagada lehe parim kasutus.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again