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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Principles of method if other than guideline:
according to Fed. Reg. 38, No. 187, § 1500.42; 1973 (Draize Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2-dimethylpropane-1,3-diamine
EC Number:
230-819-0
EC Name:
2,2-dimethylpropane-1,3-diamine
Cas Number:
7328-91-8
Molecular formula:
C5H14N2
IUPAC Name:
2,2-dimethylpropane-1,3-diamine
Details on test material:
Name of the test substance used in the study report: Neopentandiamin (= 1,3-Diamino-2,2-dimethylpropan)

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
A standardized animal laboratory diet was used.
Mean body weight: 2.8 kg

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eye of the same animal
Amount / concentration applied:
0.1 ml
Duration of treatment / exposure:
24 h
Observation period (in vivo):
8 d
Number of animals or in vitro replicates:
3
Details on study design:
scoring according to Draize

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24
Score:
3
Max. score:
3
Reversibility:
other: after 48 h, the eye was clotty (purulence) and thus could not be estimated
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24 h
Score:
1
Max. score:
1
Reversibility:
other: after 48 h, the eye was clotty (purulence) and thus could not be estimated
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24 h
Score:
2
Max. score:
2
Reversibility:
other: after 48 h, the eye was clotty (purulence) and thus could not be estimated
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 h
Score:
2
Max. score:
2
Reversibility:
not reversible

Any other information on results incl. tables

Readings

Animal

Cornea

Iris

Conjunctiva

Symptoms

op

ar

red

sw

 

24 h

1

3

4

1

2

2

 

Pupil contracted, nictitation membrane, mucosa reddish brown, blood

2

3

4

1

2

2

 

3

3

4

1

2

2

 

48 h

1

*

*

*

*

3

 

 

2

*

*

*

*

3

 

 

3

4

4

*

2

3

 

 

72 h

1

*

*

*

*

3

 

 

2

*

*

*

*

3

 

 

3

4

4

*

2

3

 

 

8 days

1

*

*

*

*

3

 

 

2

*

*

*

*

3

 

 

3

*

*

*

*

3

 

 

*eye was clotty, could not be estimated

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Conclusions:
The test substance is corrosive to eyes.