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EC number: 242-745-6
CAS number: 19009-56-4
Table 1 demonstrates the results of the test groups after 10 minutes
With the negative control (saline) neither an increase of opacity nor
permeability of the corneae could be observed (mean IVIS = 1.44). The
positive control (2-Ethoxyethanol) was tested undiluted and showed clear
opacity and distinctive permeability of the corneae (mean IVIS = 98.08)
corresponding to a classification as serious eye damaging (CLP/EPA/GHS
(Cat 1)). The test item was tested undiluted. Relative to the negative
control, the test item did not cause an increase of the corneal opacity
or permeability. The calculated mean IVIS was 1.02 (threshold for
serious eye damage: IVIS > 55). According to OECD 437, the test item is
Table 1. Results after 10 minutes incubation time
OPACITY VALUE = DIFFERENCE (t130-t0) OF OPACITY
PERMEABILITY AT 490 nm (OD490)
PROPOSED IN VITRO IRRITANCY SCORE
The test item was assessed for eye irritation as per OECD Guideline 437,
26 July 2013.
After a first opacity measurement of the fresh bovine corneae (t0), the
neat test item, the positive, and the negative controls were applied to
corneae fixed in an incubation chamber in horizontal position for 10
minutes at 32 ±1 °C. The posterior chamber contained incubation medium.
After the incubation phase the test item, the positive, and the negative
controls were each rinsed from the corneae. Further, the corneae were
incubated for another 120 minutes at 32 ±1 °C in a vertical position,
while the anterior chamber contain incubation medium as well.
Afterwards, opacity was measured a second time (t130).
After the opacity measurements permeability of the corneae was
determined by measuring spectrophotometrically the transfer of sodium
fluorescein after incubation in a horizontal position for 90 minutes at
32 ± 1 °C.
Relative to the negative control, the test item did not cause an
increase of the corneal opacity or permeability. The calculated mean
IVIS was 1.02 (threshold for serious eye damage: IVIS > 55). According
to OECD 437, the test item is not classified for eye irritation.
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