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EC number: 235-125-1
CAS number: 12070-14-3
Table 1: Test acceptance criteria
Mean Absolute OD570 nmNK
0.8 < NK < 2.5
Mean Relative Viability PC [%]
Max. Difference of % Viability [%]
The test item showed no non-specific reduction of MTT (NSMTT) and no
colouring potential. Therefore, no additional controls for correction of
results were necessary.
The controls confirmed the validity of the study. The mean absolute
OD570 of the two negative control tissues was > 0.8 and < 2.5 (1.441).
The mean relative tissue viability (% negative control) of the positive
control was < 50% (32.7%). The inter tissue difference of replicate
tissues of all dose groups was < 20% (0.4 - 3.9%).
Table 2: Results
of the Test Item Zirconium disilicide
Mean OD570ofthe duplicates(blank-corrected)
Total mean OD570of2 replicate tissues(blank-corrected)
Relative tissue viability [%]
Relative tissue viability difference [%]***
CV [% viability]
Mean relative tissue viability [%]
*= corrected mean OD570 of the negative control corresponds to
100% absolute tissue viability
**= mean relative tissue viability of the positive control is < 50%
***= relative tissue viability difference of replicate tissues is
In the present study the eye irritant potential of Zirconium
disilicide (99.47% purity) was analysed according to OECD 492 using the
three-dimensional human corneal epithelium model EpiOcular, consisting
of normal, human-derived epidermal keratinocytes mimicking
characteristics of the corneal epithelium. Hereby, 50 mg of Zirconium
disilicide applied directly atop the EpiOcular™ tissue. Cytotoxicity is
expressed as the reduction of mitochondrial dehydrogenase activity
measured by formazan production from MTT after a 6 hours exposure and
18 hours post-incubation period and compared to those of the concurrent
negative controls. Zirconium disilicide showed no non-specific reduction
of MTT and no colouring potential. Therefore, no additional controls for
correction of results were necessary. Zirconium disilicide showed no
irritant effects. The mean relative tissue viability of two replicates
(% negative control) was > 60% (115.1%). Therefore, Zirconium disilicide
is considered to be non-irritating to the eye in accordance with UN GHS
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