Reaction mass of 1-chlorodecane and 1-chlorotetraceane

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03.08.1995 to 13.10.1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.3 (Acute Toxicity (Dermal))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorotetradecane

EC Number:

219-368-0

EC Name:

1-chlorotetradecane

Cas Number:

2425-54-9

Molecular formula:

C14H29Cl

IUPAC Name:

1-chlorotetradecane

Test material form:

liquid

Details on test material:

IUCLID Test substance: as prescribed by 1.1 - 1.4

Test animals

Species:

rat

Strain:

other: Hsd/Cpb: WU (SPF)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS:
- Strain: Rat / Hsd/Cpb: WU (SPF)
- Number and sex: 10 animals (5 males and 5 females)
- Breeder: Harlan Winkelmann
- Body weigth: 200 - 300 g

ENVIRONMENTAL CONDITIONS:
- Diet: a certified commercial diet (ssniff® R 10) ad libitum
- Water: tab water ad libitum
- Housing singly in MAKROLON cages (type III)
- Temperature: 22°C ± 3°C
- Rel. Humidity: 30% - 70%
- Light/dark period: 12/12h
- Acclimation operiod: at least 5 days

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 h

Doses:

2000 mg/kg, application volume 2.3 ml/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

The exposure of the test substance was 24 hours, dermal semiocclusive  application. After 24 hours the treated skin was cleaned with corn oil.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortality. 

Clinical signs:

No systemic clinical symptoms were observed.

Body weight:

No changes in body weight gain were observed.

Gross pathology:

No macroscopic organ changes were observed.

Other findings:

Local effects: Up to 72 hours after application, slight to moderate edema  and erythema were observed. After 48 h desquamation and hardening of the skin was observed. The symptoms disappeared up to day 13 after treatment.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the described test conditions, mortality did not occur up to the highest dose tested of 2000 mg/kg/bw. Thus, the LD50 is above 2000 mg/kg/bw, and according to Regulation (EC) 1272/2008 the data are conclusive but not sufficient for classification.