Registration Dossier
Registration Dossier
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EC number: 907-242-4 | CAS number: -
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-11-28 to 1996-02-05
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- (1984)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 92/69/EEC
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 part 1
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1-chlorotetradecane
- EC Number:
- 219-368-0
- EC Name:
- 1-chlorotetradecane
- Cas Number:
- 2425-54-9
- Molecular formula:
- C14H29Cl
- IUPAC Name:
- 1-chlorotetradecane
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples was taken directly from the test vessel. Each test solutions has been analysed at 0 h and 24 h.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 1 g/L test substance in water has been stirred for 18 h and filtered.
A stability test showed that the test substance concentration is not stable after 72 h. Therefore, the test substance solution was renewed daily.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg
- Length: 3 cm +/- 0.5 cm
- Acclimation conditions: flow-through system
- Type and amount of food during acclimation: TetraMin, daily 1% of body weight
- Feeding frequency during acclimation:daily
- Treatment with malachite green (3x per week) and 14 days quarantine
- Health during acclimation (any mortality observed): healthy stock before test begin, mortality <= 5% n
- no feeding during test
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- ~11 ° dH
- Test temperature:
- 19-20 °C
- pH:
- 7.8 to 8.4
- Dissolved oxygen:
- 83 to 98 %
- Nominal and measured concentrations:
- nominal: 1 g/L, measured: 237 µg/L arith. mean
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 L filled with 10 L
- Renewal rate of test solution: daily
- aeration: continous in test vessel
- No. of organisms per vessel: 10 fish/test vessel
- No. of vessels per concentration (replicates): 4
- Biomass loading rate: 10 fish/vessel, ~0.31 g/Fisch
- Source/preparation of dilution water: drinking water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/ 8 h
EFFECT PARAMETERS MEASURED: mortality, determinde after 24 h, 48 h, 72 h, and 96 h - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.24 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The test substance concentration was not stable. Therefore the arith. mean of the test substance concentration after 0h and 24 h has been calculated. The highest attainable test substance concentration has been determined to 237 µg/L. No mortality has been observed in the control or with the test substance.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Within the highest concentration which could be obtained under the conditions of the test no mortality could be observed, thus the 96 h LC50 is > 0,24 mg/l.
All concentrations are based on the substance. The highest concentration under test conditions is 237 µg/l.
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