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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 27
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl 2-ethylhexanoate
EC Number:
221-043-3
EC Name:
Ethyl 2-ethylhexanoate
Cas Number:
2983-37-1
Molecular formula:
C10H20O2
IUPAC Name:
ethyl 2-ethylhexanoate
Details on test material:
- Name of test material (as cited in study report): 2-Ethyl-ethylcapronat
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance was directly weighed into the test vessels.

Test organisms

Details on inoculum:
no data

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min

Test conditions

Hardness:
no data
Test temperature:
no data
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
0, 10000 mg/L
Details on test conditions:
no data
TEST SYSTEM
- Aeration: no data
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
>= 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
no data
Results with reference substance (positive control):
n.a.

Any other information on results incl. tables

 

 

mg/L O2/min

Inhibition [%]

Control

1.78 (mean of 3)

n.a.

Test substance, 10000 mg/L

1.7

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Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The 30 min EC0 of 2-Ethyl ethyl capronate to Pseudomonas putida was >= 10000 mg/L
Executive summary:

In a 30 min Pseudomonas oxygen consumption inhibition test according to DIN 38412 part 27, draft, Pseudomonas putida was exposed to 2-Ethyl ethyl capronate (100% a.i.) at nominal concentrations of 0 (control) and 10000 mg/L.

No inhibition of oxygen consumption was observed in comparison to the control. The EC0 of 2-Ethyl ethyl capronate was >= 10000 mg/L