Ethyl 2-ethylhexanoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2-ethylhexanoate

EC Number:

221-043-3

EC Name:

Ethyl 2-ethylhexanoate

Cas Number:

2983-37-1

Molecular formula:

C10H20O2

IUPAC Name:

ethyl 2-ethylhexanoate

Details on test material:

- Name of test material (as cited in study report): 2-Ethyl-ethylcapronat
- Physical state: liquid
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100, 300 mg/L
- Sampling method: not described in test report

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- due to low solubility in water, the test substance was directly weighed into the test vessels and treated 10 s with ultraturrax

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Westaquarium
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Weight at study initiation (mean and range, SD): no data
- Feeding during test
- Food type: pelleted Altromin 1324
- Amount: no data
- Frequency: no data

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): no data
- Type and amount of food: pelleted Altromin 1324
- Feeding frequency:no data
- Health during acclimation (any mortality observed): mortality < 0.1% during the week before the test

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22.0 - 23.5°C

pH:

8.2 - 8.4

Dissolved oxygen:

70 - 97 mg/L in 24 h old media

Nominal and measured concentrations:

nokinal: 0, 100, 300, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria with 5 L fill volume
- Aeration: no data
- Renewal rate of test solution: 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate:< no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
mortality: 0, 6, 24, 48, 72, 96 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

173 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

not applicable

Any other information on results incl. tables

Sublethal observations / clinical signs:

Cumulative Mortality:

Conc. [mg/L]	0 h	6 h	24 h	48 h	72 h	96 h
0	0	0	0	0	0	0
100	0	0	0	0	0	0
300	0	0	100	100	100	100
1000	0	100	100	100	100	100

As the test substance is stable in water and the oxygen concentration did not decrease drastically (i.e. no biodegradation occurred) , it was assumed that the substance was present in the test system in total (either dissolved, dispersed or deposed).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 h LC50 of 2-Ethyl ethyl capronate in this acute toxicity test with Zebrafish was 173 mg/L.

Executive summary:

In a 96-h acute toxicity study according to ISO 7346 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish - Part 2: Semi-static method), Zebrafish (Brachydanio rerio; new name: Danio rerio), were exposed to 2-Ethyl ethyl capronate (100% a.i.) at nominal concentrations of 0 (control), 100, 300 and 1000 mg/L under semi-static conditions.

No mortality was observed in the control and at 100 mg/L during the 96 h test period. At 300 mg/L 10/10 fish were dead after 24 h; at 1000 mg/L 10/10 fish died within 6 h.

The 96 h LC50 was 173 mg a.i./L. 

 

Results Synopsis

Test organism: Zebrafish (Brachydanio rerio; new name: Danio rerio)

Test Type: Static Renewal

LC50:  173 mg a.i./L

Endpoint(s) Effected: mortality