3,4-dimethoxyphenethylamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Principles of method if other than guideline:

Method: other: BASF-test

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dimethoxyphenethylamine

EC Number:

204-376-9

EC Name:

3,4-dimethoxyphenethylamine

Cas Number:

120-20-7

Molecular formula:

C10H15NO2

IUPAC Name:

2-(3,4-dimethoxyphenyl)ethanamine

Test material form:

liquid

Details on test material:

- name of substance: homoveratrylamin
- Purity: 98 %
- substance number: 78/836
- appearance: clear, yellowish, slightly viscous liquid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

- source: SPF-Zucht, Hagemann, Externtal and WIGA, Sulzfeld, Germany
- mean body weights: 193 g (males) and 180 g (females).
- diet: Herilan MRH-Kraftfutter, H. Eggersmann, Rinteln/Weser, ad libitum
- water: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 50 cm²
- Type of wrap if used: inhert plastic film

REMOVAL OF TEST SUBSTANCE
- Washing (if done): warm water
- Time after start of exposure: 24 h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1,000; 1,470; 2,000; 2,610 mg/kg bw.
- For solids, paste formed: no

Duration of exposure:

24 h

Doses:

1,000; 1,470; 2,000; 2,610 mg/kg bw.

No. of animals per sex per dose:

- 5 animals per sex at the 2,610 and 1,470 mg/kg bw dose level.
- 3 animals per sex at the 1,000 mg/kg bw dose level.
- 6 animals per sex at the 2,000 mg/kg bw dose level.

Control animals:

other: Untreated part of the skin.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 2 800 mg/kg bw

Mortality:

- dead male animals at the 2,610 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/1/1/1/1
- dead female animals at the 2,610 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/1/1/3/3
- dead male animals at the 2,000 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/1/2/2/2
- dead female animals at the 2,000 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/2/2/2/2
- dead male animals at the 1,470 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/0/0/0/0
- dead female animals at the 1,470 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/1/1/1/1
- dead male animals at the 1,000 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/0/0/0/0
- dead female animals at the 1,000 mg/kg bw dose level after 1 h/ 24 h/48 h/7 days/14 days: 0/0/0/0/0

Clinical signs:

other: dyspnoe, apathy, excitation, aggressivness, staggering, tremor, twitch, spasmic gait, convulsions, deep yellow urine, piloerection, lacrimation, clotted eyes, vocalisation of pain, poor general state. Necroses at all dose levels.

Gross pathology:

- Animals that died in the course of the study: a) Heart: acute dilatation and acute venous hyperemia; b) Liver with slightly pale peripheral lobules; c) Kidney: grey-brown light-coloured kidneys (nephron necrosis).
- Animals that were sacrificed after the end of the study: no abnormalities detected.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 was ca. 2800 mg/kg bw with the test substance homoveratrylamin.