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EC number: 944-209-3
CAS number: -
biodegradation of Amides, C8-18 even numbered,
investigated in a study conducted according to OECD Guideline 301
F (Ready Biodegradability: Manometric Respirometry Test) and EU Method
C.4-D (Determination of the "Ready" Biodegradability - Manometric
Respirometry Test) over a period of 28
days and using domestic activated sludge as inoculum. The biodegradation
rate was determined by measurement of oxygen consumption. Inoculum
blank, abiotic control, procedural/functional control with the reference
substance sodium benzoate, and toxicity control using 100 mg/L test item
and 100 mg/L reference compound were performed.
The test item proved to be readily
biodegradable at a test substance concentration of 25 mg/L (81 %
biodegradation after 28 d). At a concentration of 100 mg/L, the test
substance was biodegraded by 57 % after 28 d; thus, the pass level of
60% biodegradation was not reached. However, due to the high ThOD of
2.73 mg O2/mg test substance, the test item suspension with a
concentration of 25 mg/L met the criteria of 50 -100 mg ThOD or COD/L as
demanded in the guideline.
No significant nitrification was observed
until test end (at most 7.6 % of theoretical NO3 production in the
higher concentrated assay). No abiotic degradation of the test item was
The reference item sodium benzoate was
biodegraded by 90 % after 14 days and thus fulfilling the guideline
In the toxicity control (100 mg/L test and
reference substance each) the biodegradation amounted to 62 % after 14
d. Thus, the test item can be considered as non toxic to the inoculum.
The oxygen uptake of the inoculum blank was < 60 mg/L in 28 d and the pH
was inside the range of 6.0 -8.5. The difference of extremes of
replicate values of the removal of the test item at the end of the test
was less than 20 %.
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