D-2-hydroxy-2-phenylacetic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

06-05-2002 to 21-05-2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

L-2-hydroxy-2-phenylacetic acid

EC Number:

210-276-6

EC Name:

L-2-hydroxy-2-phenylacetic acid

Cas Number:

611-71-2

Molecular formula:

C8H8O3

IUPAC Name:

hydroxy(phenyl)acetic acid

Details on test material:

ChiPros (R)-Mandelsäure: (R)-mandelic acid (purity: 100 %)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

14 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

20 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

32 mg/L

Based on:

test mat.

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

preliminary investigations (non-GLP):

- TOC = 627 mg/g
- DOC = 634 mg/g

% Degradation

Parameter:

% degradation (DOC removal)

Value:

ca. 90 - ca. 100

Sampling time:

14 d

Details on results:

- adaptation phase: 1-2 days

- degradation phase: 1-2 days

- 10-day window met: yes

Kinetic of test substance (in %):
< 0 after 1 day(s)
ca. 100 after 3 day(s)
ca. 100 after 7 day(s)
ca. 99 after 14 day(s)
Kinetic of control substance (in %):
ca. 90 ... 100 after 14 day(s)

Any other information on results incl. tables

Table 1: DOC (mg/l, average values):

 

time (d)	BC	RS	IH	PC	AC	TS
0	0.3	20	41.3	21.5	21.6	21.4
1	0.3	20.6	41.5	21.4	22	21.4
3	1.9	18.2	20.3	21.7	22.2	2.4
5	0.5	2.1	2.9	21.3	21.9	1.2
7	1.6	2.6	3	21.9		1.5
10	0.8	2.5	2.9	21.6		1.1
13	1.1	2.6	2.4	22		1.1
14	0.8	2.2	2	22		1.2

 

BC = blank control

RS = reference substance

IH = inhibition control

PC = abiotic control

AC = adsorption control

TS = test substance

 

 Table 2: DOC decrease (%):

 

time (d)	RS	IH	PC	AC	TS
0	0	0	0	0	0
1	-3	0	0	-1	-2
3	17	55	-1	5	100
5	92	94	1	0	97
7	95	97	-2	100
10	91	95	0	99
13	92	97	-2	100
14	93	97	-2	99
0	0	0	0	0	0
1	-3	0	0	-1	-2
3	17	55	-1	5	100
5	92	94	1	0	97
7	95	97	-2	100
10	91	95	0	99
13	92	97	-2	100
14	93	97	-2	99

 

BC = blank control

RS = reference substance

IH = inhibition control

PC = abiotic control

AC = adsorption control

TS = test substance

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item is readily biodegradable.The 10-day window criterion is fulfilled.

Executive summary:

An experimental study according to OECD guideline 301 A and GLP regulations was conducted to assess the biodegradability of the test item. An inoculum consisting of domestic activated sludge was exposed to an initial test item concentration of 32 mg/L (20 mg/L DOC) for 14 d under aerobic conditions. After 14 d a degradation of 90 to 100 % was observed. The 10-day criterion was met. Therefore, the test item is readily biodegradable according to OECD criteria.