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EC number: 939-478-9
CAS number: -
Table 1: Stability Test Results of Test Substance in Test Media
Analysed Concentration (ug/L)
10% stock solution
20% stock solution
40% stock solution
80% stock solution
100% stock solution
#: The average of the
anlysed concentrations of fresh and aged test solutions on days 0, 3, 9
and 12. The concentration value in this table has considered the
* ND: not detected.
Table 2: Hatching and
survival Observed in the Test Fish
(a) Initiation of
hatching on the third day; (b) The larvae in the control group began to
die from hunger at twelve days, and the test ended. The hatching rates
and survival rates were calculated based on the results of eleven days;
(c) Survival rates = survival number / hatching number x 100%.
Table 3: Abnormal Appearance and Abnormal Behaviour of Test Fish
Note: NB: normal
behavior and appearance; (a) Initiation of hatching on the third day;
(b) The larvae in the control group began to die from hunger at twelve
days, and the test ended.
Table 2: Hatching rate, survival rate and normal rate of the embryos and
larvae at the end of the test (11 d)
Mean Measured concentration (µg/L)
Adjusted hatching rate, survival rate and normal rate (%) *
EC50 (µg/L, based on mean measured concentration)
95% CI (µg/L, based on mean measured concentration)
100% stock solution (mean measured concentration 196 µg/L)
≥ 100% stock solution (mean measured concentration 196 µg/L)
* Adjusted by Abbotts,
formula. Adjusted hatching rate, survival rate and normal rate were
calculated based on the results of eleven days.
In a 12 d semi-static toxicity test on embryo and sac-fry stages
according to OECD Guideline 212 and GLP principles, fertilized eggs of
Gobiocypris rarus (rare minnow) were exposed to the test substance at
10%, 20%, 40%, 80% and 100% of stock solution (prepared at 100 mg/L
after 24h stirring and filtration) and an untreated control, in
triplicate. Mean measured concentrations were 18.5, 34.6, 78.0, 145 and
196 ug/L, respectively. Hatching, survival, abnormal appearance and
behaviour were monitored during the test and at the end of the test the
total length of the fish was assessed. Hatching rate was 93.3 - 100%,
overall survival was 93.0 - 100%. No abnormal appearance or behaviour
was observed. No significant difference in total length was observed.
The EC50 for hatching, survival and malformations was > 100% stock
solution (mean measured concentration 196 ug/L); NOEC for hatching,
survival, malformations and length was 100% stock solution (mean
measured concentration 196 ug/L). The study met all validity criteria
and is considered reliable without restriction.
NOEC: 196 ug/L, which is considered the maximum solubility of the test
substance in test medium.
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