Styrene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Concentrations of Hydrocarbons in Tissues as a Measure of Toxicity

Author:

Shugaev, B.B.

Year:

1969

Bibliographic source:

Archives of Environmental Health 18: 878-882

Reference 2

Reference Type:

secondary source

Title:

European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Author:

European Union

Year:

2007

Bibliographic source:

European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Materials and methods

Principles of method if other than guideline:

Animals were placed into a dynamic flow exposure chamber. After removal from the flow chamber, the animals were sacrificed at different times as soon as they recovered from narcotic state.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Styrene

EC Number:

202-851-5

EC Name:

Styrene

Cas Number:

100-42-5

Molecular formula:

C8H8

IUPAC Name:

ethenylbenzene

Specific details on test material used for the study:

- Name of test material (as cited in study report): Styrene
- Physical state: vapour
- Analytical purity: no data

Test animals

Species:

rat

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: no data

Analytical verification of test atmosphere concentrations:

yes

Remarks:

gas chromatography

Duration of exposure:

4 h

Concentrations:

no data

No. of animals per sex per dose:

no data

Control animals:

not specified

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LC50

Effect level:

11.8 mg/L air

95% CL:

17.8 - 24.8

Exp. duration:

4 h

Any other information on results incl. tables

Table: Concentrations of Styrene found in brain and liver of rats

Time after removal from chamber (min)	Brain (mg/100 cm3)		Liver (mg/100 cm3)
	Average of 4 experiments	Range	Average of 4 experiments	Range
1	21.8	14.8 – 30.1	20.2	11.3 – 29.7
15	22.2	13.5 – 31.6	23.5	12.9 – 32.0
30	17.7	10.7 – 22.4	19.1	14.2 – 29.0
60	8.6	3.3 – 14.7	12.8	5.7 – 20.2
90	Traces to 4.4	-	6.8	5.2 – 11.0