Formamide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Unsuitable test system. Not relevant to exposure (unphysiological pathway of application).

Data source

Reference

Title:

No information

Author:

BASF AG (1963). Department of Toxicology.|Gewerbetoxikologische Vorprüfung. Unpublished study results|(XIII/18), 26 March 1963.

Materials and methods

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Formamide

EC Number:

200-842-0

EC Name:

Formamide

Cas Number:

75-12-7

Molecular formula:

CH3NO

IUPAC Name:

formamide

Details on test material:

TS-Freetext:
Formamide, degree of purity not reported.

Test animals

Species:

mouse

Strain:

other: Hannover

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

ca. 227, 1813, 2266, 2833, 3626, 7251 mg/kg bw (0.2, 1.6, 2.0, 2.5, 3.2, 6.4 ml/kg bw)

No. of animals per sex per dose:

10

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 2 720 mg/kg bw

Any other information on results incl. tables

RS-Freetext:
Original values:
LD50 = 2.4 (2.06-2.8) ml/kg bw (related to the pure test
substance after 7 days; = 2720 (2334-3172) mg/kg bw
(calculated with a density of 1.133 g/cm3).
LD50 ca. 2.0 ml/kg bw (ca. 2266 mg/kg bw) related to the
pure test substance after 14 days.

No signs of toxicity and no deaths were observed at the
lowest dose level; these animals were sacrificed after an
observation period of 7 days (higher dose groups:
observation period of 14 days). In the other groups,
clinical signs were observed beginning on the day of dosing.
Clinical symptoms included dyspnea, ataxia, apathy,
imbalance, atony, lateral recumbent position and reduced
food consumption. The symptoms were observed immediately
after dosing (highest dose level) or at the day of dosing
(groups administered 1.6-3.2 ml/kg bw) and resolved slowly
within 11-12 days. Late deaths were observed; animals died
within 2 hours after dosing and up to 9 days after dosing.
All mice of the highest dose group were dead on the day
after dosing. Surviving rats appeared normal within 11-12
days after dosing. Autopsy revealed a fatty degeneration of
the liver in two of the animals that died. No other
pathological findings were reported.

dose level          test   deaths after
[ml/kg]  [mg/kg]    conc.  1 h    24 h   48 h    7 d  14 d
===========================================================
  0.2    ca.  227    2%    0/10   0/10   0/10   0/10   --
  1.6    ca. 1813   20%    0/10   0/10   0/10   1/10   3/10
  2.0    ca. 2266   20%    0/10   0/10   0/10   3/10   3/10
  2.5    ca. 2833   20%    0/10   0/10   1/10   1/10   1/10
  3.2    ca. 3626   30%    0/10   0/10   1/10   9/10   9/10
  6.4    ca. 7251   30%    0/10  10/10  10/10  10/10  10/10
===========================================================