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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to the appropriate OECD test guideline and in compliance with GLP. The restriction is that

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(3-chloro-2-hydroxypropyl)trimethylammonium chloride
EC Number:
222-048-3
EC Name:
(3-chloro-2-hydroxypropyl)trimethylammonium chloride
Cas Number:
3327-22-8
Molecular formula:
C6H15ClNO.Cl
IUPAC Name:
3-chloro-2-hydroxy-N,N,N-trimethylpropan-1-aminium chloride
Details on test material:

69.35 % 3-Chloro-2-hydroxypropyl)trimethylammoniumchloride, 0.2 % 2,3-Dihydroxypropyltrimethylammoniumchloride, <0.05 % 2.3-epoxypropyltrimethylammoniumchloride, 28.85 % water

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Details on test conditions:
Type: aquatic

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
1 032 mg/L
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 2 000 mg/L
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 2 000 mg/L
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)

Any other information on results incl. tables

No EC50-value could be found with the highest test concentration of 2000 mg/l. In the test report EC20-, EC50 -
and EC80-values higher than 2000 mg/l have been reported, but no EC10-value has been estimated. Based on graphical
estimation EC10-value of 1225 mg/l can be extrapolated.
There were no estimations of goodness of fit parameters available in graphs (standard deviation, R2 etc.)

In the addendum to the test report EC10-value has been estimated to be 1032 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a good quality study conducted according to OECD Test Guideline 209 and in compliance with GLP, the EC50 for respiration inhibition of activated sludge was determined to be >2000 mg/l. This result is expressed as active ingredient.