(3-chloro-2-hydroxypropyl)trimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1988-08-11 to 1988-08-12

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The test was carried out in accordance with an appropriate test method, and in compliance with GLP. No substance-specific analysis was performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

shorter exposure period, no analytical monitoring

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(3-chloro-2-hydroxypropyl)trimethylammonium chloride

EC Number:

222-048-3

EC Name:

(3-chloro-2-hydroxypropyl)trimethylammonium chloride

Cas Number:

3327-22-8

Molecular formula:

C6H15ClNO.Cl

IUPAC Name:

3-chloro-2-hydroxy-N,N,N-trimethylpropan-1-aminium chloride

Details on test material:

- Name of test material (as cited in study report): QUAB1888

- Physical state: colourless liquid

- Other: good water solubility

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: 10,000 mg of test substance were dissolved in 1L of dilution water, from which serial dilutions were made.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM

- Source: in house bred
- Age at study initiation (mean and range, SD): <24 h

- Method of breeding: as described in Nederlands praktijkrichtlijn NPR 6503 (1980)
- Feeding during test -
- Food type:
- Amount:
- Frequency:

ACCLIMATION
- Acclimation period: not reported, in house bred

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

Temperature of the test solutions was about 20 ± 1 °C

pH:

The pH of the test and control solutions varied between 7.3 and 8.0. 

Dissolved oxygen:

The oxygen concentrations of the test and control solutions were greater than 8.3 mg/l.

Nominal and measured concentrations:

Nominal concentrations: 32, 56, 100, 180, 560, 1000, 1800 and 3200 mg/L.

Details on test conditions:

TEST SYSTEM

- Test vessel: beakers

- Material, size, headspace, fill volume: 150 ml glass beakers filled with 100 ml test medium.

- Aeration: no

- No. of organisms per vessel: 5

- No. of vessels per concentration (replicates): 2

- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: synthetic water DSWL (as described in Nederlands praktijkrichtlijn NPR 6503 1980), prepared from ground water sourced near Linschoten.

- Alkalinity: expressed as hardness: 210 mg/L as CaCO3

- Ca/mg ratio: 2:1

- Culture medium different from test medium: no

- Intervals of water quality measurement: at 0 and 48 h

OTHER TEST CONDITIONS

- Adjustment of pH: not reported

- Photoperiod: 16 h light and 8 h dark cycle

- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility and condition and colour compared to control after 24 h


TEST CONCENTRATIONS

- Spacing factor for test concentrations: 1.8

- Range finding study: yes

- Test concentrations: not reported

- Results used to determine the conditions for the definitive study: not reported

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

36.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: immobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

240.2 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: immobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

650 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

other: immobility

Details on results:

- Behavioural abnormalities: It was noticed that the swimming behaviour of the animals in test solutions differed from the controls; animals swam slowly with irregular movements and closer to the bottom.  In addition, animals in test solutions were said to be paler than in controls. At a concentration of 56 mg/l (36.6 mg/l as the test substance was 65.44 %) no difference between the test solutions and controls were noticed in swimming behaviour, appearance and number of the animals. Already at a test concentration of 100 mg/l the swimming behaviour of Daphnia was different from the controls. Apparently, observations were visual, no statistical analysis was made.

- Mortality of control: 10%

Reported statistics and error estimates:

EC50 was said to be calculated by means of a parametric model developed by Kooijman.

Any other information on results incl. tables

Table 1. Number of mobile animals

Time (h)	Concentration of test substance (mg/L)
	0	32	56	100	180	320	560	1000	1 800	3 200
0	20	20	20	20	20	20	20	20	20	20
24	20	20	20	19	18	13	5	0	0	0

The results reported as whole test substance are:

24 h EC50 = 367 mg/L

24 h EC100 = 1000 mg/L

24 h NOEC = 180 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A 24 h EC50 value of 240 mg/L (active ingredient, equivalent to 360 mg/L whole substance) has been determined for the effects of QUAS 188 towards the immobility of the freshwater invertebrate D. magna.