N-(cyclohexylthio)phthalimide

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No data of positive control

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(cyclohexylthio)phthalimide

EC Number:

241-774-1

EC Name:

N-(cyclohexylthio)phthalimide

Cas Number:

17796-82-6

Molecular formula:

C14H15NO2S

IUPAC Name:

N-(cyclohexylthio)phthalimide

Details on test material:

- Name of test material (as cited in study report): N-(cyclohexylthio)phthalimede
- Substance type:monoconstituent
- Physical state:yellowish white crystalic powder
- Analytical purity: 99.7%
- Composition of test material, percentage of components:99.7%
- Lot/batch No.:GEO1
- Stability under test conditions:stable, IR spectra was used for stability check
- Storage condition of test material: lab. refrigerator
- Other: melting point: 91°C

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: daphnia magna
- Source: Environment agency, National Institute for environmental Studies
- Age of parental stock (mean and range, SD):less than 24 h old animals
- Feeding during test: yes
- Food type: Chlorella vulgaris
- Amount:0.2 mg C per a Daphnia per day


ACCLIMATION
- Acclimation period: March 22nd 2000 - April 4th 2000
- Acclimation conditions (same as test or not): same
- Type and amount of food: Chlorella vulgaris
- Amount:0.2 mg C per a Daphnia per day
- Health during acclimation (any mortality observed): 0%

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Test conditions

Hardness:

around 250 mg/L

Test temperature:

20±1°C

pH:

6-9, variation 1.5

Dissolved oxygen:

> 60% of saturated level, saturated level = 8.8mg/L at 20°C

Nominal and measured concentrations:

nominal concentration of control series: 0.100, 0.210, 0.450, 0.950, 2.00 mg/L
measured concentratin: 0.088 - 0.106 mg/L.... 88-106% of nominal
0.198 - 0.202 mg/L.....94-96%
0.396 - 0.460 mg/L.....88-102%
0.916 - 0.949 mg/L.....96-100%
1.83 - 1.99 mg/L.........92-100%
separetion factor: 2.1

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker, teflon sheet cover
- Material, size, headspace, fill volume:80 mL/vessel
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 1 animal/vessel
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt 4,


OTHER TEST CONDITIONS
- Adjustment of pH: 4x during the test period, before and after renewal
- Photoperiod: 16 h light/ 8 h dark, interior light
- Light intensity: up to 800 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : no factors detected

VEHICLE CONTROL PERFORMED: yes
conc.: 100 mg/L
RANGE-FINDING STUDY
- Test concentrations: acute toxicity 48h EC50>2.00 mg/L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: 95%CL

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified