Diisononyl adipate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

The study was performed according to European standard test guideline, Directive 79/831/EWG, appendix V, part C, stand Nov 1989.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 100 mg/L stock solution was prepared using the vehicle Cremophor RH 40 and mixing for 1020 minutes at 20 °C. The stock solution was stepwise diluted to form the desired test concentrations.
- Chemical name of vehicle: Cremophor RH 40
- Concentration of vehicle in test medium (stock solution): 100 mg/L

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture originally obtained from Institut National de Recherche Chimique Appliquee, France
- Age at study initiation (mean and range, SD): 2-26h
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.69 mmol/L

Test temperature:

20 °C

pH:

7.7 - 8.1

Dissolved oxygen:

7.6 - 9.3 mg/L

Nominal and measured concentrations:

Nominal: 0.049, 0.098, 0.195, 0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 Ml test vessels with 10 mL test solution
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: at the beginning and the end of the test (DO, pH). temperature continuously

OTHER TEST CONDITIONS
- Photoperiod: continuous dark

EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 3, 6, 24 and 48h exposure time.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: At concentrations between 1.56 and 100 mg/l the test organisms swam partly at the surface.
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none

Immobilised organisms after 48 hours (from 20 organisms / conc.):
nom. conc. (mg/l )   0h	 3h  6h	 24h 48h	
(control)            -	 -   -	 -   -   
(cont. solubilizer)  -	 -   -	 -   -   
100	             -	 -   -	 3   3  
 50                  -	 -   -	 1   1   
 25	             -   -   -   -   2   
 12.5	             -	 -   -	 -   2   
  6.25               -	 -   -	 -   2   
  3.13               -	 -   -	 -   2   
  1.56               -	 -   -	 -   1   
  0.78               -	 -   -	 -   -   
  0.39               -	 -   -	 -   -   
  0.195              -	 -   -	 -   -   
  0.098              -	 -   -	 -   -   
  0.049              -	 -   -	 -   -   

REMARK
Effect concentration is greater than the solubility of the substance in  the test medium. 

OXYGEN CONTROL (mg/l)
nom. conc. (mg/l )   0h	  48h 	
(control)            9.1  8.9   
(cont. solubilizer)  9.0  7.6   
100	             8.5  8.2
 50                  8.8  8.2 
 25	             9.0  8.2   
 12.5	             9.0  8.2   
  6.25               9.1  8.9  
  3.13               9.2  8.9
  1.56               9.2  8.9
  0.78               9.2  8.9
  0.39               9.2  8.9 
  0.195              9.3  9.0 
  0.098              9.3  9.0 
  0.049              9.3  9.0 

pH CONTROL           0h	  48h 	
(control)            8.0  7.9   
(cont. solubilizer)  8.0  7.8   
100	             7.7  7.7
 50                  7.9  7.8 
 25	             8.0  7.8   
 12.5	             8.0  7.9   
  6.25               8.0  7.9  
  3.13               8.1  7.9 
  1.56               8.0  8.0 
  0.78               8.1  7.9 
  0.39               8.1  8.0 
  0.195              8.1  8.0 
  0.098              8.1  8.0 
  0.049              8.0  7.9 

Validity criteria fulfilled:

yes

Conclusions:

In a acute toxicity test under static conditions, Daphnia magna showed no mortality to DINA concentrations up to 50 mg/L. The 48h-EC50 value is determined to be > 100 mg/L.

Description of key information

EC50 (48h) > 100 mg/L (nominal, vehicle used) for Daphnia magna (similar to OECD 202)

Additional information

One study investigating the short-term toxicity of Diisononyl adipate (DINA) to aquatic invertebrates is available. The study was performed according to European standard test guideline, Directive 79/831/EWG, appendix V, part C, adopted in Nov 1989 (similar to OECD 202) under static conditions using Daphnia magna as test organism (BASF 1990). Concentrations between 0.049 and 100 mg/L were tested using a solubilizer (Cremophor RH 40). No mortality was observed in the control and treatments up to 50 mg/L during the exposure period of 48h. The 48h-EC50 is determined to be > 100 mg/L based on nominal concentrations.