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EC number: 284-310-3
CAS number: 84852-02-8
A study was performed to assess the ability of CAS# 98072-31-2 to induce
chromosomal aberrations in human lymphocytes cultured in vitro.
Human lymphocytes, in whole blood culture, were stimulated to divide by
addition of phytohaemagglutinin, and exposed to the test substance both
in the absence and presence of S9 mix derived from rat livers. Solvent
and positive control cultures were also included. Two hours before the
end of the incubation period, cell division was arrested using
Colcemid®, the cells harvested and slides prepared, so that metaphase
cells could be examined for chromosomal damage.
In order to determine the toxicity of CAS# 98072-31-2 to cultured human
lymphocytes, the mitotic index was assessed for all cultures treated
with the test substance and the solvent control, acetone. Justification
for concentration selection was based on cytotoxicity. On the basis of
these data, the following concentrations were selected for metaphase
In the absence of S9 mix - 3 hour treatment, 18 hour recovery: 35.27,
In the presence of S9 mix (2% v/v) - 3 hour treatment, 18 hour
recovery: 58.79, 272.16 and 453.60 µg/mL.
In the absence of S9 mix - 21 hour continuous treatment: 30, 45 and 55
In the presence of S9 mix (5% v/v) - 3 hour treatment, 18 hour
recovery: 272.16, 453.60 and 1260 µg/mL.
In both the absence and presence of S9 mix, CAS# 98072-31-2 caused no
statistically significant increases in the proportion of metaphase
figures containing chromosomal aberrations, at any concentration, when
compared with the solvent control, in either test.
No statistically significant increases in the proportion of polyploid
cells were observed during metaphase analysis, in either test.
All positive control compounds caused statistically significant
increases in the proportion of aberrant cells, demonstrating the
sensitivity of the test system and the efficacy of the S9 mix.
It is concluded that CAS# 98072-31-2 has shown no evidence of causing an
increase in the frequency of structural chromosome aberrations in this
in vitro cytogenetic test system, under the experimental conditions
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